Poly(ADP-ribosyl)ation as a new posttranslational modification of YB-1

Abstract

Multifunctional Y-box binding protein 1 (YB-1) is actively studied as one of the components of cellular response to genotoxic stress. However, the precise role of YB-1 in the process of DNA repair is still obscure. In the present work we report for the first time new posttranslational modification of YB-1 - poly(ADP-ribosyl)ation, catalyzed by one of the main regulatory enzymes of DNA repair - poly(ADP-ribose)polymerase 1 (PARP1) in the presence of model DNA substrate carrying multiple DNA lesions. Therefore, poly(ADP-ribosyl)ation of YB-1 catalyzed with PARP1, can be stimulated by damaged DNA. The observed property of YB-1 underlines its ability to participate in the DNA repair by its involvement in the regulatory cascades of DNA repair.

Keywords: AP endonuclease 1 (APE1); Multiple DNA lesions; Poly(ADP-ribose) (PAR; Poly(ADP-ribose)polymerase 1 (PARP1); Y-box binding protein 1 (YB-1); pADPr).