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Case Reports
. 2015 Oct 9:10:77.
doi: 10.1186/s40793-015-0035-8. eCollection 2015.

The complete genome sequence and emendation of the hyperthermophilic, obligate iron-reducing archaeon "Geoglobus ahangari" strain 234(T)

Michael P Manzella  1 , Dawn E Holmes  2 , Jessica M Rocheleau  2 , Amanda Chung  2 , Gemma Reguera  1 , Kazem Kashefi  1
Affiliations
Case Reports

The complete genome sequence and emendation of the hyperthermophilic, obligate iron-reducing archaeon "Geoglobus ahangari" strain 234(T)

Michael P Manzella et al. Stand Genomic Sci. .

Abstract

"Geoglobus ahangari" strain 234(T) is an obligate Fe(III)-reducing member of the Archaeoglobales, within the archaeal phylum Euryarchaeota, isolated from the Guaymas Basin hydrothermal system. It grows optimally at 88 °C by coupling the reduction of Fe(III) oxides to the oxidation of a wide range of compounds, including long-chain fatty acids, and also grows autotrophically with hydrogen and Fe(III). It is the first archaeon reported to use a direct contact mechanism for Fe(III) oxide reduction, relying on a single archaellum for locomotion, numerous curled extracellular appendages for attachment, and outer-surface heme-containing proteins for electron transfer to the insoluble Fe(III) oxides. Here we describe the annotation of the genome of "G. ahangari" strain 234(T) and identify components critical to its versatility in electron donor utilization and obligate Fe(III) respiratory metabolism at high temperatures. The genome comprises a single, circular chromosome of 1,770,093 base pairs containing 2034 protein-coding genes and 52 RNA genes. In addition, emended descriptions of the genus "Geoglobus" and species "G. ahangari" are described.

Keywords: Archaeoglobales; Autotroph; Euryarchaeota; Extracellular electron transfer; Fe(III) respiration; Guaymas basin; Hydrothermal vent.

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Figures

Fig. 1
Fig. 1
Phylogenetic tree. The phylogenetic tree was constructed with the maximum likelihood algorithm comparing the16S rRNA gene sequence from “G. ahangari” to other hyperthermophilic archaea. Bootstrap values were determined from 100 replicates and “Aquifex aeolicus” and Pseudothermotoga thermarum were used as outgroups
Fig. 2
Fig. 2
Scanning electron micrograph of cells of “G. ahangari” strain 234T growing on insoluble Fe(III) oxides. Bar, 100 nm
Fig. 3
Fig. 3
Graphical circular map of the chromosome. From outside to the center: Genes on forward strand (colored by COG categories), genes on reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, and GC skew
Fig. 4
Fig. 4
Central metabolism in “Geoglobus ahangari” strain 234T
See this image and copyright information in PMC

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