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. 2015 Dec 18;51(97):17225-8.
doi: 10.1039/c5cc06817g.

A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase

D Baud  1 , N Ladkau  1 , T S Moody  2 , J M Ward  3 , H C Hailes  1
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A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase

D Baud et al. Chem Commun (Camb). .

Abstract

A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.

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