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. 2016:1358:59-69.
doi: 10.1007/978-1-4939-3067-8_4.

Studying the Translatome with Polysome Profiling

Paola Zuccotti  1 , Angelika Modelska  2
Affiliations

Studying the Translatome with Polysome Profiling

Paola Zuccotti et al. Methods Mol Biol. 2016.

Abstract

Polysome fractionation by sucrose density gradient centrifugation followed by analysis of RNA and protein is a technique that allows to understand the changes in translation of individual mRNAs as well as genome-wide effects on the translatome. Here, we describe the polysome profiling technique and RNA as well as protein isolation procedures from sucrose fractions.

Keywords: Polysome; Profiling; Protein isolation; RNA isolation; Sucrose gradient; Transcriptome; Translational control; Translatome.

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