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. 2015 Aug 1;8(8):e20215.
doi: 10.5812/jjm.20215v2. eCollection 2015 Aug.

Antibiotic Resistance Profile and Distribution of Oxacillinase Genes Among Clinical Isolates of Acinetobacter baumannii in Shiraz Teaching Hospitals, 2012 - 2013

Sara Kooti  1 , Mohammad Motamedifar  2 , Jamal Sarvari  3
Affiliations

Antibiotic Resistance Profile and Distribution of Oxacillinase Genes Among Clinical Isolates of Acinetobacter baumannii in Shiraz Teaching Hospitals, 2012 - 2013

Sara Kooti et al. Jundishapur J Microbiol. .

Abstract

Background: The emergence of multidrug-resistant Acinetobacter baumannii complicates the therapy of the related infections. Hospital isolates of A. baumannii are usually multidrug-resistant. The problem is compounded by increasing resistance to broad-spectrum antibiotics including carbapenems.

Objectives: The aim of this study was to determine antimicrobial susceptibility patterns and distribution of bla OXA -type carbapenemases genes among A. baumannii isolates from hospitalized patients in Shiraz, Southwest Iran.

Materials and methods: Two hundred A. baumannii isolates were recovered from different clinical specimens in four Shiraz teaching hospitals. Isolates were detected as A. baumannii by Microgen kit and PCR with specific primers of bla OXA-51-like gene. Antimicrobial susceptibility testing was determined by disk diffusion method for all the isolates. Multiplex PCR assays were performed for detection of bla OXA-23-like , bla OXA-24-like and bla OXA-58-like genes.

Results: All the isolates were susceptible to colistin and polymyxin B. Moreover, all of them were resistant to piperacillin, piperacillin-tazobactam, ampicillin, ceftazidime, cefoxitin and aztreonam. Eighty (40%) isolates had positive results for bla OXA-23-like , 14 (7%) for bla OXA-24-like and 1 (0.5%) isolate for bla OXA-58-like . The co-existence of studied genes was detected for bla OXA-23-like plus bla OXA-24-like in nine (4.5%) isolates.

Conclusions: The prevalence of carbapenem resistant A. baumannii isolates in Shiraz hospitals is high. The bla OXA-23-like gene was the most frequent carbapenemase identified among resistant A. baumannii isolated in Shiraz hospitals. The increasing incidence of A. baumannii is a serious concern, therefore control of this pathogen and taking preventive measures are emphasized.

Keywords: Acinetobacter baumannii; OXA-51 beta-lactamase; Oxacillinase.

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Figures

Figure 1.
Figure 1.. Agarose Gel Electrophoresis of blaOXA-51-like Gene in Acinetobacter baumannii
1, 2, 3, 4, 5, clinical isolates of A. baumannii with blaOXA-51-like gene; C-, negative control; C+, positive control; M, 100 bp DNA ladder.
Figure 2.
Figure 2.. Agarose Gel Electrophoresis of blaOXA-type Carbapenemases by Multiplex PCR
1, clinical isolate of A. baumannii containing blaOXA-23-like and blaOXA-24-like genes; 2, clinical isolate of A. baumannii having blaOXA-58-like gene; 3,4, clinical isolates of A. baumannii having blaOXA-23-like genes; 5, clinical isolate of A. baumannii with blaOXA-24-like gene; C-, negative control; C+, positive control (A. baumannii NCTC 13304, NCTC 13302, NCTC 13305 for blaOXA-23-like, blaOXA-24-like, blaOXA-58-like ); M, 100 bp DNA ladder.
See this image and copyright information in PMC

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