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Comparative Study
. 1989 Mar 10;243(4896):1363-6.
doi: 10.1126/science.2646717.

Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase

J R Sampson  1 , A B DiRenzo, L S Behlen, O C Uhlenbeck
Affiliations
Comparative Study

Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase

J R Sampson et al. Science. .

Abstract

An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.

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