A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome

Abstract

A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the genome was estimated to 136 kbp for the Morocco isolate (S1MNPV-M2) and to 135 kbp for the Lyon isolate (S1MNPV-L4). HindIII-K was used as the zero-point of the map because it hybridized to EcoRI-I and BamHI-F fragment of Autographa californica (AcMNPV) DNA, and the direction of transcription of the S1MNPV polyhedrin gene was chosen left to right. Hybridization of six AcMNPV cloned DNA fragments with S1MNPV-B genome shows that S1MNPV-B and AcMNPV genomic structures are not aligned.