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Review
. 2016 Mar 1:96:69-74.
doi: 10.1016/j.ymeth.2015.10.005. Epub 2015 Oct 17.

TransgeneOmics--A transgenic platform for protein localization based function exploration

Susanne Hasse  1 , Anthony A Hyman  1 , Mihail Sarov  1
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Free article
Review

TransgeneOmics--A transgenic platform for protein localization based function exploration

Susanne Hasse et al. Methods. .
Free article

Abstract

The localization of a protein is intrinsically linked to its role in the structural and functional organization of the cell. Advances in transgenic technology have streamlined the use of protein localization as a function discovery tool. Here we review the use of large genomic DNA constructs such as bacterial artificial chromosomes as a transgenic platform for systematic tag-based protein function exploration.

Keywords: BAC transgenesis; Genome engineering; Protein localization; Protein tagging; Recombineering.

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