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. 1989 Jan;9(1):329-31.
doi: 10.1128/mcb.9.1.329-331.1989.

A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo

M Winey  1 , I Edelman, M R Culbertson
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A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo

M Winey et al. Mol Cell Biol. 1989 Jan.

Abstract

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

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