Review
doi: 10.1007/978-1-4939-3079-1_7.
Translation Analysis at the Genome Scale by Ribosome Profiling
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- PMID: 26483019
- DOI: 10.1007/978-1-4939-3079-1_7
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Review
Translation Analysis at the Genome Scale by Ribosome Profiling
Methods Mol Biol.
2016.
Abstract
Ribosome profiling is an emerging approach using deep sequencing of the mRNA part protected by the ribosome to study protein synthesis at the genome scale. This approach provides new insights into gene regulation at the translational level. In this review we describe the protocol to prepare polysomes and extract ribosome protected fragments before to deep sequence them.
Keywords: Recoding; Ribo-seq; Ribosome profiling; Translation regulation.
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