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. 1989 Apr;86(7):2281-5.
doi: 10.1073/pnas.86.7.2281.

Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease

J Heitman  1 , N D Zinder, P Model
Affiliations

Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease

J Heitman et al. Proc Natl Acad Sci U S A. 1989 Apr.

Abstract

We prepared a set of temperature-sensitive mutants of the EcoRI endonuclease. Under semipermissive conditions, Escherichia coli strains bearing these alleles form poorly growing colonies in which intracellular substrates are cleaved at EcoRI sites and the SOS DNA repair response is induced. Strains defective in SOS induction (lexA3 mutant) or SOS induction and recombination (recA56 and recB21 mutants) are not more sensitive to this in vivo DNA scission, whereas strains deficient in DNA ligase (lig4 and lig ts7 mutants) are extremely sensitive. We conclude that although DNA scission induces the SOS response, neither this induction nor recombination are required for repair. DNA ligase is necessary and may be sufficient to repair EcoRI-mediated DNA breaks in the E. coli chromosome.

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References

    1. J Mol Biol. 1973 Jul 15;77(4):531-47 - PubMed
    1. J Mol Biol. 1973 Jul 15;77(4):519-29 - PubMed
    1. Gene. 1982 Jul-Aug;19(1):1-10 - PubMed
    1. J Biol Chem. 1983 Jan 25;258(2):711-3 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2310-3 - PubMed

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