Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jan 20:3:143-5.
doi: 10.1016/j.gdata.2015.01.004. eCollection 2015 Mar.

Transcriptome analysis of response to drought in poplar interspecific hybrids

Elena Barghini  1 , Rosa Maria Cossu  1 , Andrea Cavallini  1 , Tommaso Giordani  1
Affiliations

Transcriptome analysis of response to drought in poplar interspecific hybrids

Elena Barghini et al. Genom Data. .

Abstract

To investigate the response of poplar hybrids to drought, leaves were collected from plants to which water was suspended for 8 and 13 days. After measuring the respective relative water content, RNAs were isolated from leaves of moderately and severely droughted plants and from control plants, and Illumina RNA sequencing was performed to analyze RNA synthesis in these tissues. Our data provide a resource (available at Gene Expression Omnibus database under GSE64044) to be employed for comparative analyses of drought response in different poplar species, with the long-term aim of developing strategies to improve plant productivity under drought.

Keywords: Drought; Gene expression; Illumina; Poplar; RNAseq.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Leaves (yellow circles) of P. deltoides × P. nigra plants used in the experiments. From left to right: normally watered, moderately, and severely droughted plants. For each leaf is reported the relative water content.
Fig. 2
Fig. 2
Schematic representation of nine gene expression patterns (indicated by letters (a)–(i)) observed in leaves of poplar hybrids comparing control conditions (C) to moderate (D1) and to severe drought (D2). For each pattern, the number of genes is reported. Only expressed genes (i.e., with at least one read per million reads aligned in at least one sample) were counted.
See this image and copyright information in PMC

References

    1. Logemann J., Schell J., Willmitzer L. Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 1987;163:16–20. - PubMed
    1. Bolger A.M., Lohse M., Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30:2114–2120. - PMC - PubMed
    1. Tuskan G.A., Difazio S., Jansson S., Bohlmann J., Grigoriev I., Hellsten U., Putnam N., Ralph S., Rombauts S., Salamov A. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray) Science. 2006;313:1596–1604. - PubMed
    1. Sterck L., Rombauts S., Jansson S., Sterky F., Rouzé P., Van de Peer Y. EST data suggest that poplar is an ancient polyploid. New Phytol. 2005;167:165–170. - PubMed
    1. Mortazavi A., Williams B.A., McCue K., Schaeffer L., Wold B. Mapping and quantifying mammalian transcriptomes by RNA-seq. Nat. Methods. 2008;5:621–628. - PubMed

LinkOut - more resources