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. 2015 Sep 18:6:150.
doi: 10.4103/2152-7806.165765. eCollection 2015.

The effects of sodium-2-mercaptoethanesulfonate application on the neural and neurovascular tissues: An experimental animal study

Ayca Ant  1 , Recep Karamert  2 , Gamze Kulduk  3 , Özgür Ekinci  3 , Hakan Tutar  2 , Nebil Göksu  2
Affiliations

The effects of sodium-2-mercaptoethanesulfonate application on the neural and neurovascular tissues: An experimental animal study

Ayca Ant et al. Surg Neurol Int. .

Abstract

Background: Sodium-2-mercaptoethanesulfonate (MESNA) is a protective agent that is also used as "a chemical dissector" in various surgical fields. The aim of this study is to evaluate the toxic effects of MESNA on neural and neurovascular structures based on a morphological analysis and examine its safety in neurotological applications.

Methods: Three groups of guinea pigs were used as subjects. MESNA solution (50 and 100%) and saline solution were applied to the subarachnoid space over the brain tissue via a middle fossa approach of study and control groups, respectively. Effects of MESNA were assessed by means of light microscope. McNemar Chi-square test was used to evaluate the histopathological findings. Statistical significance of P < 0.05 was taken as criterion.

Results: No morphological changes were observed on vascular and neural structures in the study groups in both concentrations, compared to the control group.

Conclusions: On a morphological basis, a single application of MESNA does not cause any morphological changes that indicate a toxicity in neural and neurovascular structures.

Keywords: Neurotologic surgery; neurotoxicity; sodium-2-mercaptoethanolsulfonate.

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Figures

Figure 1
Figure 1
Surgical procedure; skin incision for the procedure
Figure 2
Figure 2
Surgical procedure; exposure of the zygomatic root (star)
Figure 3
Figure 3
Surgical procedure; craniotomy above the zygomatic root, dura flap with the Penrose drain
Figure 4
Figure 4
Surgical procedure; injection to the subarachnoid space
Figure 5
Figure 5
Histologic section of study-1 group (H and E, ×10). No morphological change was seen in each group
Figure 6
Figure 6
Histologic section of study-1 group (H and E, ×40). No morphological change of arteriole and venule (a), neuron (b), capillary (c) was seen in each group
Figure 7
Figure 7
Histologic section of study-2 group (H and E, ×10). No morphological change was seen in each group
Figure 8
Figure 8
Histologic section of study-2 group (H and E, ×40). No morphological change of arteriole and venule (a), neuron (b), capillary (c) was seen in each group
Figure 9
Figure 9
Histologic section of control group (H and E, ×10). No morphological change was seen in each group
Figure 10
Figure 10
Histologic section of control group (H and E, ×40). No morphological change of arteriole and venule (a), neuron (b), capillary (c) was seen in each group
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