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. 2015 Nov;21(11):2045-7.
doi: 10.3201/eid2111.150750.

Fosfomycin Resistance in Escherichia coli, Pennsylvania, USA

Hind Alrowais, Christi L McElheny, Caressa N Spychala, Sangeeta Sastry, Qinglan Guo, Adeel A Butt, Yohei Doi

Fosfomycin Resistance in Escherichia coli, Pennsylvania, USA

Hind Alrowais et al. Emerg Infect Dis. 2015 Nov.

Abstract

Fosfomycin resistance in Escherichia coli is rare in the United States. An extended-spectrum β-lactamase-producing E. coli clinical strain identified in Pennsylvania, USA, showed high-level fosfomycin resistance caused by the fosA3 gene. The IncFII plasmid carrying this gene had a structure similar to those found in China, where fosfomycin resistance is commonly described.

Keywords: 16S rRNA methyltransferase; ESBL; Escherichia coli; Pennsylvania; United States; antimicrobial resistance; bacteria; extended-spectrum β-lactamase; fosA3 gene; fosfomycin resistance; glutathione S-transferase; plasmids.

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Figures

Figure 1
Figure 1
Comparative analysis of fosA3-carrying IncFII plasmids pYHCC with pXZ. Open reading frames are indicated by arrows and colored according to their putative functions: magenta arrows indicate genes involved in replication; blue arrows indicate genes associated with plasmid conjugal transfer. Brown arrows indicate genes involved in plasmid stability; red arrows indicate antimicrobial drug resistance genes; green arrows indicate accessory genes of mobile elements; gray arrows indicate other backbone genes and inserted foreign genes. Light blue shading indicates shared backbone regions with a high degree of homology. MRR, multidrug resistance region.
Figure 2
Figure 2
EcoRI restriction profile of fosA3-carrying plasmids from a woman in Pennsylvania, USA, who was colonized with fosfomycin-resistant Escherichia coli. Lanes M, lambda DNA/HindIII marker; lane 1, February 2011 (YD472); lane 2, March 2008; lane 3, June 2007 (ECRB1); lane 4, April 2008; lane 5, January 2008. Values on the left are in kilobases.
See this image and copyright information in PMC

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