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. 2016 Jan;54(1):178-9.
doi: 10.1128/JCM.01754-15. Epub 2015 Oct 21.

Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study

Choon-Mee Kim  1 , Min Keun Cho  2 , Dong-Min Kim  3 , Na-Ra Yun  2 , Seok Won Kim  4 , Sook Jin Jang  5 , Young-Joon Ahn  6 , Donghoon Lim  7
Affiliations

Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study

Choon-Mee Kim et al. J Clin Microbiol. 2016 Jan.

Abstract

We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy.

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