doi: 10.1128/jb.171.4.2241-2243.1989.
Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids
Affiliations
- PMID: 2649493
- PMCID: PMC209887
- DOI: 10.1128/jb.171.4.2241-2243.1989
Item in Clipboard
Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids
J Bacteriol.
1989 Apr.
Abstract
Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.
Similar articles
-
xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase.EMBO J. 1989 May;8(5):1623-7. doi: 10.1002/j.1460-2075.1989.tb03547.x. EMBO J. 1989. PMID: 2670557 Free PMC article.
-
The arginine repressor is essential for plasmid-stabilizing site-specific recombination at the ColE1 cer locus.EMBO J. 1988 Dec 20;7(13):4389-95. doi: 10.1002/j.1460-2075.1988.tb03338.x. EMBO J. 1988. PMID: 3149585 Free PMC article.
-
Multicopy plasmid stability in Escherichia coli requires host-encoded functions that lead to plasmid site-specific recombination.Mol Gen Genet. 1988 Sep;214(1):80-4. doi: 10.1007/BF00340183. Mol Gen Genet. 1988. PMID: 3067082
-
Timing, self-control and a sense of direction are the secrets of multicopy plasmid stability.Mol Microbiol. 1998 Sep;29(5):1137-45. doi: 10.1046/j.1365-2958.1998.01012.x. Mol Microbiol. 1998. PMID: 9767582 Review.
-
DNA synthesis of plasmids in Escherichia coli.Biotechnol Genet Eng Rev. 1992;10:209-52. doi: 10.1080/02648725.1992.10647889. Biotechnol Genet Eng Rev. 1992. PMID: 1284510 Review. No abstract available.
Cited by
-
Low-level expression of the Type II restriction-modification system confers potent bacteriophage resistance in Escherichia coli.DNA Res. 2020 Feb 1;27(1):dsaa003. doi: 10.1093/dnares/dsaa003. DNA Res. 2020. PMID: 32167561 Free PMC article.
-
Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases.J Bacteriol. 1990 Dec;172(12):6973-80. doi: 10.1128/jb.172.12.6973-6980.1990. J Bacteriol. 1990. PMID: 2254268 Free PMC article.
-
Site-specific recombination between ColE1 cer and NTP16 nmr sites in vivo.Mol Gen Genet. 1995 May 20;247(4):509-14. doi: 10.1007/BF00293154. Mol Gen Genet. 1995. PMID: 7770060
-
Quantifying complexity in DNA structures with high resolution Atomic Force Microscopy.Nat Commun. 2025 Jul 1;16(1):5482. doi: 10.1038/s41467-025-60559-x. Nat Commun. 2025. PMID: 40593613 Free PMC article.
References
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
