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. 2015 Oct 24;15(1):150.
doi: 10.1093/jisesa/iev132. Print 2015.

Genetic variation and geographic differentiation among populations of the nonmigratory agricultural pest Oedaleus infernalis (Orthoptera: Acridoidea) in China

Wei Sun  1 , Hui Dong  2 , Yue-Bo Gao  3 , Qian-Fu Su  1 , Hai-Tao Qian  2 , Hong-Yan Bai  4 , Zhu-Ting Zhang  5 , Bin Cong  6
Affiliations

Genetic variation and geographic differentiation among populations of the nonmigratory agricultural pest Oedaleus infernalis (Orthoptera: Acridoidea) in China

Wei Sun et al. J Insect Sci. .

Abstract

The nonmigratory grasshopper Oedaleus infernalis Saussure (Orthoptera : Acridoidea) is an agricultural pest to crops and forage grasses over a wide natural geographical distribution in China. The genetic diversity and genetic variation among 10 geographically separated populations of O. infernalis was assessed using polymerase chain reaction-based molecular markers, including the intersimple sequence repeat and mitochondrial cytochrome oxidase sequences. A high level of genetic diversity was detected among these populations from the intersimple sequence repeat (H: 0.2628, I: 0.4129, Hs: 0.2130) and cytochrome oxidase analyses (Hd: 0.653). There was no obvious geographical structure based on an unweighted pair group method analysis and median-joining network. The values of FST, θ(II), and Gst estimated in this study are low, and the gene flow is high (Nm > 4). Analysis of the molecular variance suggested that most of the genetic variation occurs within populations, whereas only a small variation takes place between populations. No significant correlation was found between the genetic distance and geographical distance. Overall, our results suggest that the geographical distance plays an unimpeded role in the gene flow among O. infernalis populations.

Keywords: COI; ISSR; gene flow; genetic diversity.

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Figures

Fig. 1.
Fig. 1.
Sample location of O. infernalis in China.
Fig. 2.
Fig. 2.
UPGMA dendrogram for the 10 populations of O. infernalis.
Fig. 3.
Fig. 3.
Mantel test between geographical distance and genetic distance among different populations of O. infernalis based on ISSR data.
Fig. 4.
Fig. 4.
Median-joining haplotype network of O. infernalis based on COI gene of mtDNA. The circle areas are proportional to haplotypes frequencies, while the color portions represent the proportions of the same haplotype occurring in each sampling region.
Fig. 5.
Fig. 5.
Mantel test between geographical distance and genetic distance among different populations of O. infernalis based on the COI gene.
See this image and copyright information in PMC

References

    1. Abbot P. 2001. Individual and population variation in invertebrates revealed by inter-simple sequence repeats (ISSRs). J. Insect Sci. 1: 8. - PMC - PubMed
    1. Avise J. C. 1991. Ten unorthodox perspectives on evolution prompted by comparative population genetic findings on mitochondrial DNA. Annu. Rev. Genet. 25: 45–69. - PubMed
    1. Avise J. C. 2004. Molecular markers: natural history and evolution, 2nd ed Sinauer, Sunderland, MA.
    1. Bandelt H. J., Forster P., Rohl A. 1999. Median-joining networks for inferring intraspecific phylogenies. Mol. Biol. Evol. 16: 37–48. - PubMed
    1. Berthier K., Loiseau A., Streiff R., Arlettaz R. 2008. Eleven polymorphic microsatellite markers for Oedaleus decorus (Orthoptera, Acrididae), an endangered grasshopper in Central Europe. Mol. Ecol. Resour. 8: 1363–1366. - PubMed

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