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. 2016:1365:83-97.
doi: 10.1007/978-1-4939-3124-8_4.

Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells

Derek A Applewhite  1 , Christine A Davis  2 , Eric R Griffis  3 , Omar A Quintero  4
Affiliations

Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells

Derek A Applewhite et al. Methods Mol Biol. 2016.

Abstract

In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches as well as high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.

Keywords: Actin; Electron microscopy; Fluorescence microscopy; Microtubule; S2 cells; Transfection.

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