Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015:2015:258941.
doi: 10.1155/2015/258941. Epub 2015 Oct 4.

Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts

Andreas S Gkogkos  1 , Ioannis K Karoussis  1 , Ioannis D Prevezanos  1 , Kleopatra E Marcopoulou  1 , Kyriaki Kyriakidou  1 , Ioannis A Vrotsos  1
Affiliations

Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts

Andreas S Gkogkos et al. Int J Dent. 2015.

Abstract

Aim. To evaluate the effect of Low Level Laser Therapy (LLLT) on human gingival fibroblasts in terms of proliferation and growth factors' secretion (EGF, bFGF, and VEGF). Materials and Methods. Primary cultures of keratinized mucosa fibroblasts were irradiated by a Nd:YAG laser 1064 nm with the following energy densities: 2.6 J/cm(2), 5.3 J/cm(2), 7.9 J/cm(2), and 15.8 J/cm(2). Controls were not irradiated. Cultures were examined for cell proliferation and growth factors' secretion after 24, 48, and 72 hours. All experimental procedures were performed in duplicate. Data were analyzed by Student's t-test (p < 0.05). Results. All laser-irradiation doses applied promoted a higher cell proliferation at 48 hours in a dose-response relationship compared to controls. This difference reached statistical significance for the cultures receiving 15.8 J/cm(2) (p = 0.03). Regarding EGF, all laser irradiation doses applied promoted a higher secretion at 48 hours in a reverse dose-response pattern compared to controls. This difference reached statistical significance for the cultures receiving 2.6 J/cm(2) (p = 0.04). EGF levels at the other time points, bFGF, and VEGF showed a random variation between the groups. Conclusion. Within the limits of this study, LLLT (Nd:YAG) may induce gingival fibroblasts' proliferation and upregulate the secretion of EGF. Further studies are needed to confirm these results.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The irradiation was carried out with manipolo per terapia N4060 handpiece, approximately 5 mm above cell level.
Figure 2
Figure 2
Human gingival fibroblasts at 48 hours. (a) Irradiated with Nd:YAG laser for 120 (120 s group) (original magnification, ×100). (b) Nonirradiated (Ctrl group), (original magnification, ×100).
Figure 3
Figure 3
Boxplot of EGF values differences from baseline for each radiation time and control, after 24, 48, and 72 hours.
Figure 4
Figure 4
Cells' number of control and test groups 24, 48, and 72 hours after irradiation.
See this image and copyright information in PMC

References

    1. Carroll J. D., Milward M. R., Cooper P. R., Hadis M., Palin W. M. Developments in low level light therapy (LLLT) for dentistry. Dental Materials. 2014;30(5):465–475. doi: 10.1016/j.dental.2014.02.006. - DOI - PubMed
    1. Gao X., Xing D. Molecular mechanisms of cell proliferation induced by low power laser irradiation. Journal of Biomedical Science. 2009;16(1, article 4) doi: 10.1186/1423-0127-16-4. - DOI - PMC - PubMed
    1. Safavi S. M., Kazemi B., Esmaeili M., Fallah A., Modarresi A., Mir M. Effects of low-level He-Ne laser irradiation on the gene expression of IL-1β, TNF-α, IFN-γ, TGF-β, bFGF, and PDGF in rat's gingiva. Lasers in Medical Science. 2008;23(3):331–335. doi: 10.1007/s10103-007-0491-5. - DOI - PubMed
    1. Janis J. E., Kwon R. K., Lalonde D. H. A practical guide to wound healing. Plastic and Reconstructive Surgery. 2010;125(6):230e–244e. doi: 10.1097/PRS.0b013e3181d9a0d1. - DOI - PubMed
    1. Holmes D. I. R., Zachary I. C. Vascular endothelial growth factor regulates stanniocalcin-1 expression via neuropilin-1-dependent regulation of KDR and synergism with fibroblast growth factor-2. Cellular Signalling. 2008;20(3):569–579. doi: 10.1016/j.cellsig.2007.11.009. - DOI - PubMed