Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats

Abstract

Ethnopharmacological relevance: Acorn obtained from the Quercus brantii Lindl. (QB) tree has been used in Turkish folk medicine. Some studies have reported as an antioxidant, antimicrobial, anti-inflammatory, gastroprotective, antitumoural of QB properties previously. however, its effect on the management of type diabetic 2 and oxidative stress complications is still unexplored. The aims of our study were the evaluation of the protective effect and antioxidant role of acorn lyophilized seed plant extract against STZ-induced diabetic complications as oxidative stress, hepatotoxicity and nephropathy, lipidemia and serum biomarkers of diabetes (SBD).

Materials and methods: In-vivo studies were performed on STZ-induced diabetic rats Experiment was designed as I [Normal Control (NC)], II [Diabetes mellitus (DM)], III [DM+Acarbose (20mg/kg b.w) (DM+AC-20)], IV [DM+QB (100mg/kg b.w) (DM+QB-100)], V [DM+QB (250 mg/kg b.w) (DM+QB-250)] and VI [DM+QB (500 mg/kg b.w) (DM+QB-500)] groups.

Results: This study showed that the biochemical analysis showed a considerable increase in the HRDB, DB, LP, MDA and fluctuated ADSC in the II group as compared to that of control group whereas, AC and the plant lyophilized seed plant extract supplementations diet restored the STZ-induced diabetic complications towards the control. α-glycosidase activity in DM group showed statistically significant increase with respect to control group in small intestine. Moreover, in accordance with the effects of seed extract; in diabetic rat groups to whom acorn seed extract and acarbose were given, the levels of almost all the concerned parameters were reached to the ones measured at control group. As a result, it was concluded that acorn seed extract had certain healing effects on many complications caused by diabetes.

Keywords: 1-chloro-2,4-dinitrobenzene (CDNB) (PubChem CID: 6); 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) (PubChem CID: 6254); Acorn; Antioxidant defense system; Butylated hydroxytoluene (BHT) (PubChem CID: 31404); Diabetes mellitus; Ethylenediaminetetraacetic acid (EDTA) (PubChem CID: 6049); Malondialdehyde; Oxidative stress; Oxidized glutathione (GSSG) (PubChem CID: 65359); Rats; Reduced glutathione (GSH) (PubChem CID: 124886); Serum biomarkers; Streptozotocin (STZ) (PubChem CID: 29327); Thiobarbituric acid (TBA) (PubChem CID: 2723628); Trichloroacetic acid (TCA) (PubChem CID: 6421); β-Nicotinamide adenine dinucleotide phosphate (NADPH) (PubChem CID: 5884).