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. 2015 Oct 13;8(Suppl 2):9-14.
doi: 10.4137/MBI.S31445. eCollection 2015.

Enumeration of Organohalide Respirers in Municipal Wastewater Anaerobic Digesters

Bryan Jk Smith  1 , Melissa A Boothe  1 , Brice A Fiddler  1 , Tania M Lozano  1 , Russel K Rahi  1 , Mark J Krzmarzick  1
Affiliations

Enumeration of Organohalide Respirers in Municipal Wastewater Anaerobic Digesters

Bryan Jk Smith et al. Microbiol Insights. .

Abstract

Organohalide contaminants such as triclosan and triclocarban have been well documented in municipal wastewater treatment plants (WWTPs), but the degradation of these contaminants is not well understood. One possible removal mechanism is organohalide respiration by which bacteria reduce the halogenated compound. The purpose of this study was to determine the abundance of organohalide-respiring bacteria in eight WWTP anaerobic digesters. The obligate organohalide respiring Dehalococcoides mccartyi was the most abundant and averaged 3.3 × 10(7) copies of 16S rRNA genes per gram, while the Dehalobacter was much lower at 2.6 × 10(4) copies of 16S rRNA genes per gram. The genus Sulfurospirillum spp. was also detected at 1.0 × 10(7) copies of 16S rRNA genes per gram. No other known or putatively organohalide-respiring strains in the Dehalococcoidaceae family were found to be present nor were the genera Desulfitobacterium or Desulfomonile.

Keywords: Dehalobacter; Dehalococcoides mccartyi; reductive dehalogenation; triclocarban; triclosan.

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Figures

Figure 1
Figure 1
The averages and ranges of 16S rRNA genes per gram quantified with qPCR in eight anaerobic digester samples for bacteria, Dehalobacter spp., D. mccartyi, and Sulfurospirillum spp.
See this image and copyright information in PMC

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