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Editorial
. 2015 Nov;8(11):1341-3.
doi: 10.1242/dmm.023606.

Into the blue: the importance of murine lacZ gene expression profiling in understanding and treating human disease

Chris Armit  1
Affiliations
Editorial

Into the blue: the importance of murine lacZ gene expression profiling in understanding and treating human disease

Chris Armit. Dis Model Mech. 2015 Nov.

Abstract

The International Mouse Phenotyping Consortium (IMPC) is a major international effort to explore the effects of knocking out 20,000 genes in the mouse. A new study by White and colleagues, published in the current issue of Disease Models & Mechanisms, demonstrates the usefulness of lacZ in situ reporter expression patterns in extending our understanding of genotype-phenotype relationships as part of the IMPC high-throughput screen. In situ gene expression profiling is invaluable for evaluating compartment-specific gene expression patterns, and these enrich our understanding of the role of genes in a great number of biological processes in multiple organ systems. Furthermore, the complexity of gene expression patterns informs our understanding of how genes influence lethality. This Editorial aims to highlight ways in which the lacZ expression profiles can impact on biomedical research by uncovering as-yet-unknown genotype-phenotype relationships, and through predicting the role of genes in health and disease.

Keywords: Gene expression; Mouse; lacZ.

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Conflict of interest statement

Competing interests

The author declares no competing or financial interests.

Comment on

  • A gene expression resource generated by genome-wide lacZ profiling in the mouse.
    Tuck E, Estabel J, Oellrich A, Maguire AK, Adissu HA, Souter L, Siragher E, Lillistone C, Green AL, Wardle-Jones H, Carragher DM, Karp NA, Smedley D, Adams NC; Sanger Institute Mouse Genetics Project; Bussell JN, Adams DJ, Ramírez-Solis R, Steel KP, Galli A, White JK. Tuck E, et al. Dis Model Mech. 2015 Nov;8(11):1467-78. doi: 10.1242/dmm.021238. Epub 2015 Aug 20. Dis Model Mech. 2015. PMID: 26398943 Free PMC article.

References

    1. Abu-Asab M., Amri H. and Micozzi M. S. (2013). Avicenna's Medicine: A New Translation of the 11th-Century Canon with Practical Applications for Integrative Health Care. Book IV: Diseases Not Specific to a Single Organ, 1st edn Healing Arts Press: Rochester, VT.
    1. Barker N., van Es J. H., Kuipers J., Kujala P., van den Born M., Cozijnsen M., Haegebarth A., Korving J., Begthel H., Peters P. J. et al. (2007). Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 449, 1003-1007. 10.1038/nature06196 - DOI - PubMed
    1. Barker N., Huch M., Kujala P., van de Wetering M., Snippert H. J., van Es J. H., Sato T., Stange D. E., Begthel H., van den Born M. et al. (2010). Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro. Cell Stem Cell 6, 25-36. 10.1016/j.stem.2009.11.013 - DOI - PubMed
    1. Blake J. A., Christie K. R., Dolan M. E., Drabkin H. J., Hill D. P., Ni L., Sitnikov D., Burgess S., Buza T., Gresham C. et al. (2015). Gene Ontology Consortium: going forward. Nucleic Acids Res. 43, D1049-D1056. 10.1093/nar/gku1179 - DOI - PMC - PubMed
    1. Elezaby A., Sverdlov A. L., Tu V. H., Soni K., Luptak I., Qin F., Liesa M., Shirihai O. S., Rimer J., Schaffer J. E. et al. (2015). Mitochondrial remodeling in mice with cardiomyocyte-specific lipid overload. J. Mol. Cell Cardiol. 79, 275-283. 10.1016/j.yjmcc.2014.12.001 - DOI - PMC - PubMed