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. 1989 May;171(5):2609-13.
doi: 10.1128/jb.171.5.2609-2613.1989.

Chromosomal transformation of Escherichia coli recD strains with linearized plasmids

C B Russell  1 , D S Thaler, F W Dahlquist
Affiliations

Chromosomal transformation of Escherichia coli recD strains with linearized plasmids

C B Russell et al. J Bacteriol. 1989 May.

Abstract

Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.

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References

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