New technologies for DNA analysis--a review of the READNA Project

doi:10.1016/j.nbt.2015.10.003

Review

. 2016 May 25;33(3):311-30.

doi: 10.1016/j.nbt.2015.10.003. Epub 2015 Oct 26.

New technologies for DNA analysis--a review of the READNA Project

Steven McGinn¹, David Bauer², Thomas Brefort³, Liqin Dong², Afaf El-Sagheer⁴, Abdou Elsharawy⁵, Geraint Evans⁶, Elin Falk-Sörqvist⁷, Michael Forster⁸, Simon Fredriksson⁹, Peter Freeman¹⁰, Camilla Freitag¹¹, Joachim Fritzsche¹², Spencer Gibson¹⁰, Mats Gullberg⁹, Marta Gut¹³, Simon Heath¹³, Isabelle Heath-Brun¹³, Andrew J Heron¹⁴, Johannes Hohlbein⁶, Rongqin Ke¹⁵, Owen Lancaster¹⁰, Ludovic Le Reste⁶, Giovanni Maglia¹⁴, Rodolphe Marie¹⁶, Florence Mauger¹, Florian Mertes¹⁷, Marco Mignardi¹⁵, Lotte Moens⁷, Jelle Oostmeijer¹⁸, Ruud Out¹⁸, Jonas Nyvold Pedersen¹⁶, Fredrik Persson¹¹, Vincent Picaud¹⁹, Dvir Rotem¹⁴, Nadine Schracke³, Jennifer Sengenes¹, Peer F Stähler³, Björn Stade⁸, David Stoddart¹⁴, Xia Teng¹⁸, Colin D Veal¹⁰, Nathalie Zahra¹⁰, Hagan Bayley¹⁴, Markus Beier³, Tom Brown²⁰, Cees Dekker²¹, Björn Ekström⁹, Henrik Flyvbjerg¹⁶, Andre Franke⁸, Simone Guenther²², Achillefs N Kapanidis⁶, Jane Kaye²³, Anders Kristensen¹⁶, Hans Lehrach¹⁷, Jonathan Mangion²², Sascha Sauer¹⁷, Emile Schyns²⁴, Jörg Tost¹, Joop M L M van Helvoort¹⁸, Pieter J van der Zaag²⁵, Jonas O Tegenfeldt²⁶, Anthony J Brookes¹⁰, Kalim Mir², Mats Nilsson¹⁵, James P Willcocks²⁷, Ivo G Gut²⁸

Affiliations

¹ CEA - Centre National de Génotypage, 2, rue Gaston Cremieux, 91057 Evry Cedex, France.
² The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK.
³ Comprehensive Biomarker Center GmbH, Im Neuenheimer Feld 583, D-69120 Heidelberg, Germany.
⁴ School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK; Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Rd, Oxford OX1 3TA, UK; Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt.
⁵ Institute of Clinical Molecular Biology, Christian-Albrechts-University (CAU), Am Botanischen Garten 11, D-24118 Kiel, Germany; Faculty of Sciences, Division of Biochemistry, Chemistry Department, Damietta University, New Damietta City, Egypt.
⁶ Biological Physics Research Group, Clarendon Laboratory, Department of Physics, Parks Road, Oxford OX1 3PU, UK.
⁷ Department of Immunology, Genetics, and Pathology, Science for Life Laboratory, Uppsala University, Sweden.
⁸ Institute of Clinical Molecular Biology, Christian-Albrechts-University (CAU), Am Botanischen Garten 11, D-24118 Kiel, Germany.
⁹ Olink AB, Dag Hammarskjölds väg 52A, 752 37 Uppsala, Sweden.
¹⁰ University of Leicester, University Road, Leicester LE1 7RH, UK.
¹¹ Department of Physics, University of Gothenburg, SE-412 96 Gothenburg, Sweden.
¹² Department of Applied Physics, Chalmers University of Technology, Kemivägen 10, 412 96 Göteborg, Sweden.
¹³ Centro Nacional de Análisis Genómico (CNAG-CRG), Center for Genomic Regulation, C/Baldiri Reixac 7, 08028 Barcelona, Spain; Universitat Pompeu Fabra (UPF), Barcelona, Spain.
¹⁴ Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, England, UK.
¹⁵ Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Box 1031, Se-171 21 Solna, Sweden; Department of Immunology, Genetics, and Pathology, Science for Life Laboratory, Uppsala University, Sweden.
¹⁶ DTU Nanotech, Oerstedsplads Building 345 East, 2800, Kongens Lyngby, Denmark.
¹⁷ Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany.
¹⁸ FlexGen BV, Galileiweg 8, 2333 BD Leiden, The Netherlands.
¹⁹ CEA-Saclay, Bât DIGITEO 565 - Pt Courrier 192, 91191 Gif-sur-Yvette Cedex, France.
²⁰ School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK; Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Rd, Oxford OX1 3TA, UK.
²¹ Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands.
²² Thermo Fisher Scientific Frankfurter Straße 129B, 64293 Darmstadt, Germany.
²³ HeLEX - Centre for Health, Law and Emerging Technologies, Nuffield Department of Population Health, University of Oxford, Old Road Campus, Oxford OX3 7LF, UK.
²⁴ PHOTONIS France S.A.S. Avenue Roger Roncier, 19100 Brive B.P. 520, 19106 BRIVE Cedex, France.
²⁵ Philips Research Laboratories, High Tech Campus 11, 5656 AE Eindhoven, The Netherlands.
²⁶ Division of Solid State Physics and NanoLund, Lund University, Box 118, 22100 Lund, Sweden.
²⁷ Oxford Nanopore Technologies, Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford OX4 4GA, UK.
²⁸ Centro Nacional de Análisis Genómico (CNAG-CRG), Center for Genomic Regulation, C/Baldiri Reixac 7, 08028 Barcelona, Spain; Universitat Pompeu Fabra (UPF), Barcelona, Spain. Electronic address: ivo.gut@cnag.crg.eu.

PMID: 26514324
DOI: 10.1016/j.nbt.2015.10.003

Review

New technologies for DNA analysis--a review of the READNA Project

Steven McGinn et al. N Biotechnol. 2016.

. 2016 May 25;33(3):311-30.

doi: 10.1016/j.nbt.2015.10.003. Epub 2015 Oct 26.

Authors

Affiliations

¹ CEA - Centre National de Génotypage, 2, rue Gaston Cremieux, 91057 Evry Cedex, France.
² The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK.
³ Comprehensive Biomarker Center GmbH, Im Neuenheimer Feld 583, D-69120 Heidelberg, Germany.
⁴ School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK; Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Rd, Oxford OX1 3TA, UK; Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt.
⁵ Institute of Clinical Molecular Biology, Christian-Albrechts-University (CAU), Am Botanischen Garten 11, D-24118 Kiel, Germany; Faculty of Sciences, Division of Biochemistry, Chemistry Department, Damietta University, New Damietta City, Egypt.
⁶ Biological Physics Research Group, Clarendon Laboratory, Department of Physics, Parks Road, Oxford OX1 3PU, UK.
⁷ Department of Immunology, Genetics, and Pathology, Science for Life Laboratory, Uppsala University, Sweden.
⁸ Institute of Clinical Molecular Biology, Christian-Albrechts-University (CAU), Am Botanischen Garten 11, D-24118 Kiel, Germany.
⁹ Olink AB, Dag Hammarskjölds väg 52A, 752 37 Uppsala, Sweden.
¹⁰ University of Leicester, University Road, Leicester LE1 7RH, UK.
¹¹ Department of Physics, University of Gothenburg, SE-412 96 Gothenburg, Sweden.
¹² Department of Applied Physics, Chalmers University of Technology, Kemivägen 10, 412 96 Göteborg, Sweden.
¹³ Centro Nacional de Análisis Genómico (CNAG-CRG), Center for Genomic Regulation, C/Baldiri Reixac 7, 08028 Barcelona, Spain; Universitat Pompeu Fabra (UPF), Barcelona, Spain.
¹⁴ Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, England, UK.
¹⁵ Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Box 1031, Se-171 21 Solna, Sweden; Department of Immunology, Genetics, and Pathology, Science for Life Laboratory, Uppsala University, Sweden.
¹⁶ DTU Nanotech, Oerstedsplads Building 345 East, 2800, Kongens Lyngby, Denmark.
¹⁷ Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany.
¹⁸ FlexGen BV, Galileiweg 8, 2333 BD Leiden, The Netherlands.
¹⁹ CEA-Saclay, Bât DIGITEO 565 - Pt Courrier 192, 91191 Gif-sur-Yvette Cedex, France.
²⁰ School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK; Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Rd, Oxford OX1 3TA, UK.
²¹ Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands.
²² Thermo Fisher Scientific Frankfurter Straße 129B, 64293 Darmstadt, Germany.
²³ HeLEX - Centre for Health, Law and Emerging Technologies, Nuffield Department of Population Health, University of Oxford, Old Road Campus, Oxford OX3 7LF, UK.
²⁴ PHOTONIS France S.A.S. Avenue Roger Roncier, 19100 Brive B.P. 520, 19106 BRIVE Cedex, France.
²⁵ Philips Research Laboratories, High Tech Campus 11, 5656 AE Eindhoven, The Netherlands.
²⁶ Division of Solid State Physics and NanoLund, Lund University, Box 118, 22100 Lund, Sweden.
²⁷ Oxford Nanopore Technologies, Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford OX4 4GA, UK.
²⁸ Centro Nacional de Análisis Genómico (CNAG-CRG), Center for Genomic Regulation, C/Baldiri Reixac 7, 08028 Barcelona, Spain; Universitat Pompeu Fabra (UPF), Barcelona, Spain. Electronic address: ivo.gut@cnag.crg.eu.

PMID: 26514324
DOI: 10.1016/j.nbt.2015.10.003

Abstract

The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 41/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3(rd) and 4(th) generation of sequencing methods with nanopores and in situ sequencing, respectively.

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New technologies for DNA analysis--a review of the READNA Project

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New technologies for DNA analysis--a review of the READNA Project

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