Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Sep;110(6):745-54.
doi: 10.1590/0074-02760150123.

Detection of Oropouche virus segment S in patients and inCulex quinquefasciatus in the state of Mato Grosso, Brazil

Belgath Fernandes Cardoso  1 , Otacília Pereira Serra  1 , Letícia Borges da Silva Heinen  1 , Nayara Zuchi  1 , Victor Costa de Souza  2 , Felipe Gomes Naveca  2 , Marcelo Adriano Mendes dos Santos  3 , Renata Dezengrini Slhessarenko  1
Affiliations

Detection of Oropouche virus segment S in patients and inCulex quinquefasciatus in the state of Mato Grosso, Brazil

Belgath Fernandes Cardoso et al. Mem Inst Oswaldo Cruz. 2015 Sep.

Abstract

This study aimed to investigate the circulation of Orthobunyavirus species in the state of Mato Grosso (MT) Brazil. During a dengue outbreak in 2011/2012, 529 serum samples were collected from patients with acute febrile illness with symptoms for up to five days and 387 pools of female Culex quinquefasciatus captured in 2013 were subjected to nested-reverse transcription-polymerase chain reaction for segment S of the Simbu serogroup followed by nucleotide sequencing and virus isolation in Vero cells. Patients (5/529; 0.9%) from Cuiabá (n = 3), Várzea Grande (n = 1) and Nova Mutum (n = 1) municipalities were positive for the S segment of Oropouche virus (OROV). Additionally, eight/387 Cx. quinquefasciatus pools were positive for the segment, with a minimum infection rate of 2.3. Phylogenetic analysis indicated that all the samples belong to the subgenotype Ia, presenting high homology with OROV strains obtained from humans and animals in the Brazilian Amazon. The present paper reports the first detection of an Orthobunyavirus, possibly OROV, in patients and in Cx. quinquefasciatus mosquitoes in MT. This finding reinforces the notion that arboviruses frequently reported in the Amazon Region circulate sporadically in MT during dengue outbreaks.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1. : distribution of the samples included in the study and the five patients with acute febrile illness positive for the segment S of Oropouche virus (OROV) by nested-reverse transcription-polymerase chain reaction per city of residency in the state of Mato Grosso, Brazil. Brazilian states: AM: Amazonas; GO: Goiás; MS: Mato Grosso do Sul; PA: Pará; RO: Rondônia; TO: Tocantins.
Fig. 2
Fig. 2. : distribution of Culex quinquefasciatus pools captured and positive for the segment S of Oropouche virus (OROV) according to the censitary map in Cuiabá, state of Mato Grosso, Brazil.
Fig. 3
Fig. 3. : phylogenetic tree of Oropouche virus nucleoprotein partial sequences obtained from humans (KP954633, KP347671-KP347674) and fromCulex quinquefasciatus (KP310500-KP310507) in the state of Mato Grosso and reference strains from genotypes Ia-c, IIa-c, IIIa-b and IV of the virus outgroup: Buttonwillow (KF697162), Faceys Paddock (KF697136), Ingwavuma (JQ029991), Mermet (KF697152), Aino (M22011), Tinaroo (AB000819) and Akabane (NC009896) viruses. The tree was obtained through neighbour-joining method with bootstrap of 1,000 replicates. The distance was calculated by the transition/transversion rate, Tamura three-parameter method and gamma distribution (distribution parameter gamma = 1). ●: human samples; ▲: arthropod pools.
Fig. 4
Fig. 4. : alignment of the deduced amino acid (aa) nucleoprotein partial sequence of Oropouche virus (OROV) obtained from Culex quinquefasciatus pools (OROV/MT_CbaAR) and humans (OROV/MT_CbaH and OROV/MT_NMH) with acute febrile illness from the state of Mato Grosso, Brazil compared to reference strains available at GenBank database. Sites with aa substitutions are highlighted according to their position.
See this image and copyright information in PMC

References

    1. Acrani GO, Gomes R, Proença-Módena JL, da Silva AF, Carminati PO, Silva ML, Santos RIM, Arruda E. Apoptosis induced by Oropouche virus infection in HeLa cells is dependent on virus protein expression. Virus Res. 2010;149:56–63. - PubMed
    1. Aguilar PV, Barret AD, Saeed MF, Watts DM, Russel K, Guevara C, Ampuero JS, Suárez L, Cespedes M, Montgomery JM, Halsey ES, Kochel TJ. Iquitos virus: a novel reassortant Orthobunyavirus associated with human illness in Peru. PLoS Negl Trop Dis. 2011;5: - PMC - PubMed
    1. Anderson CR, Spence L, Downs WG, Aitken TG. Oropouche virus: a new human disease agent from Trinidad, West Indies. Am J Trop Med Hyg. 1961;10:574–578. - PubMed
    1. Barbosa MD, Fé NF, Marcião AH, Silva AP, Monteiro WM, Guerra MV, Guerra JA. Record of epidemiologically important Culicidae in the rural area of Manaus, Amazonas. Rev Soc Bras Med Trop. 2008;41:658–663. - PubMed
    1. Bastos MS, Figueiredo LTM, Naveca FG, Monte RL, Lessa N, Figueiredo RMP, Gimaque JBL, João GP, Ramasawmy R, Mourão MPG. Identification of Oropouche Orthobunyavirus in the cerebrospinal fluid of three patients in the Amazonas, Brazil. Am J Trop Med Hyg. 2012;86:732–735. - PMC - PubMed

Publication types

MeSH terms