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. 1989 Feb;9(2):823-7.
doi: 10.1128/mcb.9.2.823-827.1989.

Individual protein-binding domains of the insulin gene enhancer positively activate beta-cell-specific transcription

O Karlsson  1 , M D Walker, W J Rutter, T Edlund
Affiliations

Individual protein-binding domains of the insulin gene enhancer positively activate beta-cell-specific transcription

O Karlsson et al. Mol Cell Biol. 1989 Feb.

Abstract

A beta-cell-specific enhancer is located in the 5'-flanking DNA of the rat insulin 1 gene. Two homologous 8-base-pair sequences in the enhancer (IEB1 and IEB2) significantly stimulated transcription from a heterologous promoter (two- to fourfold) in a cell-specific fashion. When the elements were combined or duplicated, more than 50% of the activity of the intact enhancer was obtained. These two cis-acting elements appear to play a dominant role in the positive control of beta-cell-specific transcription of the insulin gene.

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References

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