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. 2015 Dec;12(12):1129-31.
doi: 10.1038/nmeth.3652. Epub 2015 Nov 2.

Thermal proteome profiling monitors ligand interactions with cellular membrane proteins

Friedrich B M Reinhard  1 , Dirk Eberhard  1 , Thilo Werner  1 , Holger Franken  1 , Dorothee Childs  1   2 , Carola Doce  1 , Maria Fälth Savitski  1 , Wolfgang Huber  2 , Marcus Bantscheff  1 , Mikhail M Savitski  1 , Gerard Drewes  1
Affiliations

Thermal proteome profiling monitors ligand interactions with cellular membrane proteins

Friedrich B M Reinhard et al. Nat Methods. 2015 Dec.

Abstract

We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-binding transmembrane proteins. We also observed cellular thermal shifts in pervanadate-induced T cell-receptor signaling, delineating the membrane target CD45 and components of the downstream pathway, and with drugs affecting the transmembrane transporters ATP1A1 and MDR1.

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  • Hitting the target.
    Leestemaker Y, Ovaa H. Leestemaker Y, et al. Nat Methods. 2015 Dec;12(12):1127-8. doi: 10.1038/nmeth.3660. Nat Methods. 2015. PMID: 26624797 No abstract available.

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