Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015;88(2):175-80.
doi: 10.15386/cjmed-419. Epub 2015 Apr 15.

Photodynamic therapy of melanoma using new, synthetic porphyrins and phthalocyanines as photosensitisers - a comparative study

Affiliations

Photodynamic therapy of melanoma using new, synthetic porphyrins and phthalocyanines as photosensitisers - a comparative study

Ioana Baldea et al. Clujul Med. 2015.

Abstract

Melanoma, a cancer that arises from melanocytes, is one of the most unresponsive cancers to known therapies and has a tendency to produce early metastases. Several studies showed encouraging results of the efficacy of photodynamic therapy (PDT) in melanoma, in different experimental settings in vitro and in vivo, as well as several clinical reports.

Aims: Our study focuses on testing the antimelanoma efficacy of several new, synthetic photosensitisers (PS), from two different chemical classes, respectively four porphyrins and six phthalocyanines.

Methods: These PS were tested in terms of cell toxicity and phototoxicity against a radial growth phase melanoma cell line (WM35), in vitro. Cells were exposed to different concentrations of the PS for 24h, washed, then irradiatied with red light (630 nm) 75 mJ/cm(2) for the porphyrins and 1 J/cm(2) for the phthalocyanines. Viability was measured using the MTS method.

Results: Two of the synthetic porphyrins, TTP and THNP, were active photosensitizers against WM35 melanoma in vitro. Phthalocyanines were effective in producing a dose dependent PDT-induced decrease in viability in a dose-dependent manner. The most efficient was Indium (III) Phthalocyanine chloride, a metal substituted phthalocyanine.

Conclusions: The most efficient photosensitizers for PDT in melanoma cells were the phthalocyanines in terms of tumor cell photokilling and decreased dark toxicity.

Keywords: cell photokilling; melanoma; photodyamic therapy; phthalocyanines; porphyrins.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Chemical structures of the porphyrins tested as PS in PDT against melanoma.
Figure 2
Figure 2
Chemical structures of the six phthalocyanines tested as PS in PDT against melanoma.
Figure 3
Figure 3
Cell viability testing following PDT mediated by the four porphyrins. Viability data are presented as OD490, TTP and THNP (upper panels) proved to be good PS against WM 35 melanoma cells.
Figure 4
Figure 4
Cell viability testing following PDT mediated by the six phthalocyanines. Viability data are presented as OD490, all Pc induced photokilling with no dark toxicity, Pc1 showed the best phototoxic efficacy against WM 35 melanoma cells.

Similar articles

Cited by

References

    1. NCCN Guidelines Version 4. 2014 Melanoma. 2014. Available from: http://www.nccn.org/professionals/physician_gls/pdf/melanoma.pdf.
    1. Balch CM, Gershenwald JE, Soong SJ, Thompson JF, Atkins MB, Byrd DR, et al. Final version of 2009 AJCC melanoma staging and classification. J Clin Oncol. 2009;27(36):6199–6206. - PMC - PubMed
    1. Monge-Fuentes V, Muehlmann LA, de Azevedo RB. Perspectives on the application of nanotechnology in photodynamic therapy for the treatment of melanoma. Nano Rev. 2014;5 - PMC - PubMed
    1. Maduray K, Karsten A, Odhav B, Nyokong T. In vitro toxicity testing of zinc tetrasulfophthalocyanines in fibroblast and keratinocyte cells for the treatment of melanoma cancer by photodynamic therapy. J Photochem Photobiol B. 2011;103(2):98–104. - PubMed
    1. Robertson CA, Abrahamse H, Evans D. The in vitro PDT efficacy of a novel metallophthalocyanine (MPc) derivative and established 5-ALA photosensitizing dyes against human metastatic melanoma cells. Lasers Surg Med. 2010;42(10):766–776. - PubMed

LinkOut - more resources