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. 2015 Oct 14:6:868.
doi: 10.3389/fpls.2015.00868. eCollection 2015.

Role of Trichoderma harzianum in mitigating NaCl stress in Indian mustard (Brassica juncea L) through antioxidative defense system

Parvaiz Ahmad  1 , Abeer Hashem  2 , Elsayed Fathi Abd-Allah  3 , A A Alqarawi  3 , Riffat John  4 , Dilfuza Egamberdieva  5 , Salih Gucel  6
Affiliations

Role of Trichoderma harzianum in mitigating NaCl stress in Indian mustard (Brassica juncea L) through antioxidative defense system

Parvaiz Ahmad et al. Front Plant Sci. .

Abstract

Salinity stress affected crop production of more than 20% of irrigated land globally. In the present study the effect of different concentrations of NaCl (0, 100, and 200 mM) on growth, physio-biochemical attributes, antioxidant enzymes, oil content, etc. in Brassica juncea and the protective role of Trichoderma harzianum (TH) was investigated. Salinity stress deteriorates growth, physio-biochemical attributes, that ultimately leads to decreased biomass yield in mustard seedlings. Higher concentration of NaCl (200 mM) decreased the plant height by 33.7%, root length by 29.7% and plant dry weight (DW) by 34.5%. On the other hand, supplementation of TH to NaCl treated mustard seedlings showed elevation by 13.8, 11.8, and 16.7% in shoot, root length and plant DW respectively as compared to plants treated with NaCl (200 mM) alone. Oil content was drastically affected by NaCl treatment; however, TH added plants showed enhanced oil percentage from 19.4 to 23.4% in the present study. NaCl also degenerate the pigment content and the maximum drop of 52.0% was recorded in Chl. 'a'. Enhanced pigment content was observed by the application of TH to NaCl treated plants. Proline content showed increase by NaCl stress and maximum accumulation of 59.12% was recorded at 200 mM NaCl. Further enhancement to 70.37% in proline content was recorded by supplementation of TH. NaCl stress (200 mM) affirms the increase in H2O2 by 69.5% and MDA by 36.5%, but reduction in the accumulation is recorded by addition of TH to mustard seedlings. 200 mM NaCl elevated SOD, POD, APX, GR, GST, GPX, GSH, and GSSG in the present study. Further enhancement was observed by the application of TH to the NaCl fed seedlings. NaCl stress suppresses the uptake of important elements in both roots and shoots, however, addition of TH restored the elemental uptake in the present study. Mustard seedlings treated with NaCl and TH showed restricted Na uptake as compared to seedlings treated with NaCl alone. In conclusion, TH proved to be very beneficial in imparting resistance to the mustard plants against NaCl stress through improved uptake of essential elements, modulation of osmolytes and antioxidants.

Keywords: H2O2; NaCl; Trichoderma harzianum (TH); antioxidants; growth; lipid peroxidation; mustard; osmolytes.

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Figures

FIGURE 1
FIGURE 1
Effect on oil content (%) under NaCl stress in presence and absence of Trichoderma harzianum (TH) in Brassica juncea seedlings. Different letters indicate significant difference between means at p < 0.05 (DMRT). Values are means ± SE (n = 5).
FIGURE 2
FIGURE 2
Effect on (A) hydrogen peroxide (H2O2) and (B) malondialdehyde content (MDA) under NaCl stress in presence and absence of TH in B. juncea seedlings. Different letters indicate significant difference between means at p < 0.05. Values are means ± SE (n = 5).
FIGURE 3
FIGURE 3
Effect on (A) superoxide dismutase (SOD) and (B) peroxidase activity (POD) under NaCl stress in presence and absence of TH in B. juncea seedlings. Different letters indicate significant difference between means at p < 0.05. Values are means ± SE (n = 5).
FIGURE 4
FIGURE 4
Effect on (A) ascorbate peroxidase (APX), (B) monodehydroascorbate reductase (MDHAR), (C) dehydroascorbate reductase (DHAR), and (D) glutathione reductase (GR) under NaCl stress in presence and absence of TH in B. juncea seedlings. Different letters indicate significant difference between means at p < 0.05. Values are means ± SE (n = 5).
FIGURE 5
FIGURE 5
Effect on (A) glutathione S-transferase (GST), (B) gaucol peroxidase (GPX), and (C) catalase (CAT) activity under NaCl stress in presence and absence of TH in B. juncea seedlings. Different letters indicate significant difference between means at p < 0.05 (DMRT). Values are means ± SE (n = 5).
FIGURE 6
FIGURE 6
Effect on (A) Ascorbic acid (nmol g-1 FW), (B) GSH (Reduced glutathione) (nmol g-1 FW), (C) GSSG (Oxidized glutathione) (nmol g-1 FW) and (D) GSH/GSSG ratio under NaCl stress in presence and absence of TH in B. juncea seedlings. Different letters indicate significant difference between means at p < 0.05 (DMRT). Values are means ± SE (n = 5).
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