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. 2016 Jan;83(1):6-7.
doi: 10.1002/mrd.22595. Epub 2015 Nov 23.

High-throughput sexing of mouse blastocysts by real-time PCR using dissociation curves

Andrew M Prantner  1 , Teri Ord  1 , Sergey Medvedev  1 , George L Gerton  1   2   3
Affiliations

High-throughput sexing of mouse blastocysts by real-time PCR using dissociation curves

Andrew M Prantner et al. Mol Reprod Dev. 2016 Jan.

Abstract

Sex ratio is defined as the proportion of males to females in a population. Subdivisions of the sex ratio are the primary (ratio at fertilization) and the secondary (ratio at birth). The expected secondary sex ratio is 0.5, but biological, environmental, or occupational variables can shift the secondary sex ratio from this expectation.

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Figures

Figure 1
Figure 1
Sexing of mouse blastocysts. Representative quantitative real-time PCR melting curve data (Kdm5c/Kdm5d simplex primer set) and agarose gel data (Sry and Il2 duplex primer set) for a (A) male and (B) female blastocyst.
See this image and copyright information in PMC

References

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