Long-range translational coupling in the rplJL-rpoBC operon of Escherichia coli

Abstract

In Escherichia coli the genes encoding ribosomal proteins L10 and L7/L12, rplJ and rplL, are cotranscribed, and translation of both cistrons is regulated by binding of L10 or a complex of L10 and L7/L12 to a single target in the mRNA leader region. Co-ordinated regulation is assured by some kind of translational coupling, the mechanism of which was investigated here by deletion analysis of plasmids carrying either the intact rplL gene or rplL-lacZ gene fusions. Unless the rplL ribosome binding site was modified by deletion, efficient initiation of translation required translation of a region located more than 500 nucleotides upstream on the transcript within the rplJ cistron. It is proposed that the wild-type rplL ribosome binding site is blocked by long-range RNA base-pairing to this region, when translation of the rplJ sequence is inhibited.