Cardiomyocytes Replicate and their Numbers Increase in Young Hearts

Nawazish Naqvi¹, Reena Singh², Siiri E Iismaa³, Ming Li³, John W Calvert⁴, David I K Martin⁵, Richard P Harvey², Robert M Graham⁶, Ahsan Husain⁷

Affiliations

¹ Department of Medicine, Emory University, Atlanta, Georgia 30322, USA.
² Development and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia.
³ Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia.
⁴ Department of Surgery, Emory University, Atlanta, Georgia 30322, USA.
⁵ Children's Hospital Oakland Research Institute, Oakland, California 94609, USA.
⁶ Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia. Electronic address: b.graham@victorchang.edu.
⁷ Department of Medicine, Emory University, Atlanta, Georgia 30322, USA. Electronic address: ahusai2@emory.edu.

PMID: 26544928
PMCID: PMC5875184
DOI: 10.1016/j.cell.2015.10.038

Comment

Cardiomyocytes Replicate and their Numbers Increase in Young Hearts

Nawazish Naqvi et al. Cell. 2015.

. 2015 Nov 5;163(4):783-4.

doi: 10.1016/j.cell.2015.10.038.

Authors

Nawazish Naqvi¹, Reena Singh², Siiri E Iismaa³, Ming Li³, John W Calvert⁴, David I K Martin⁵, Richard P Harvey², Robert M Graham⁶, Ahsan Husain⁷

Affiliations

¹ Department of Medicine, Emory University, Atlanta, Georgia 30322, USA.
² Development and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia.
³ Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia.
⁴ Department of Surgery, Emory University, Atlanta, Georgia 30322, USA.
⁵ Children's Hospital Oakland Research Institute, Oakland, California 94609, USA.
⁶ Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia. Electronic address: b.graham@victorchang.edu.
⁷ Department of Medicine, Emory University, Atlanta, Georgia 30322, USA. Electronic address: ahusai2@emory.edu.

PMID: 26544928
PMCID: PMC5875184
DOI: 10.1016/j.cell.2015.10.038

No abstract available

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Figures

**Figure 1**
(A and B) Immunohistochemical identification of mitotic CMs (red staining, Aurora B-positive cells) in transverse cut tissue sections showing localization in the left ventricle (LV) of P15 mice (B), with no Aurora B-positive cells evident in the LV of the P10 heart (A). CMs are labeled with cardiac troponin T (cTNT) (green) and nuclei are labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Arrows show some Aurora B-positive CMs. Experimental procedures used for immunohistochemistry were essentially as described (Naqvi et al., 2014). The Aurora B antibody (Abcam, ab 2254), supplied as a 1-mg/ml solution, was used at a dilution of 1:50. Sections were prepared and antibodies provided by the Graham laboratory. Immunohistochemical staining was performed in a blinded manner by the Harvey laboratory.

See this image and copyright information in PMC

Comment on

A proliferative burst during preadolescence establishes the final cardiomyocyte number.
Naqvi N, Li M, Calvert JW, Tejada T, Lambert JP, Wu J, Kesteven SH, Holman SR, Matsuda T, Lovelock JD, Howard WW, Iismaa SE, Chan AY, Crawford BH, Wagner MB, Martin DI, Lefer DJ, Graham RM, Husain A. Naqvi N, et al. Cell. 2014 May 8;157(4):795-807. doi: 10.1016/j.cell.2014.03.035. Cell. 2014. PMID: 24813607 Free PMC article.
Cardiomyocyte Cell-Cycle Activity during Preadolescence.
Soonpaa MH, Zebrowski DC, Platt C, Rosenzweig A, Engel FB, Field LJ. Soonpaa MH, et al. Cell. 2015 Nov 5;163(4):781-2. doi: 10.1016/j.cell.2015.10.037. Cell. 2015. PMID: 26544927 No abstract available.
No Evidence for Cardiomyocyte Number Expansion in Preadolescent Mice.
Alkass K, Panula J, Westman M, Wu TD, Guerquin-Kern JL, Bergmann O. Alkass K, et al. Cell. 2015 Nov 5;163(4):1026-36. doi: 10.1016/j.cell.2015.10.035. Cell. 2015. PMID: 26544945

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Cardiomyocytes Replicate and their Numbers Increase in Young Hearts

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Cardiomyocytes Replicate and their Numbers Increase in Young Hearts

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Grants and funding

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