Assay for CDP-Diacylglycerol Generation by CDS in Membrane Fractions

Abstract

CDP-DAG is a liponucleotide formed by the condensation of CTP with the phospholipid phosphatidic acid in a reaction catalyzed by CDP-DAG synthase (CDS). CDP-DAG is required for the synthesis of phosphatidylinositol; the parent molecule whence all seven phosphoinositides including the signaling molecules PI4P, PI(4,5)P2, and PI(3,4,5)P3 are derived. This protocol describes a highly sensitive radiometric assay to detect the generation of CDP-DAG on isolated biological membrane fractions.

Keywords: Assay; CDP-DAG; CDS; Endoplasmic reticulum; Liponucleotide; Membrane.