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. 2016:1380:135-50.
doi: 10.1007/978-1-4939-3197-2_11.

In Vitro and In Vivo Imaging of Fluorescent Aptamers

Ioanna Théodorou  1   2   3 , Nam Nguyen Quang  1   2   3 , Karine Gombert  2   3   4 , Benoit Thézé  2   3   4 , Benoit Lelandais  1   2   3 , Frédéric Ducongé  5   6   7
Affiliations

In Vitro and In Vivo Imaging of Fluorescent Aptamers

Ioanna Théodorou et al. Methods Mol Biol. 2016.

Abstract

Fluorescence imaging techniques could be used in different ways to study the interaction of aptamers with biological systems from cell culture to animal models. Here, we present the methods developed in our laboratory for fluorescently labeled aptamers, study their internalization inside living cells using time-lapse microscopy, and monitor their biodistribution in mice bearing subcutaneous xenograft tumors using planar fluorescence imaging and fluorescence diffuse optical tomography (fDOT).

Keywords: Aptamer; Fluorescence; Fluorescence diffuse optical tomography; Oligonucleotide; Time-lapse microscopy.

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