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Comparative Study
. 1989;151(2):91-4.
doi: 10.1007/BF00414419.

The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170

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Comparative Study

The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170

C Kato et al. Arch Microbiol. 1989.

Abstract

The lipo-penicillinase (LIPEN) gene from an alkalophilic Bacillus sp. strain 170 was cloned in Escherichia coli using the vector pHSG399. A plasmid, pFAP121, was isolated from an ampicillin resistant transformant and the cloned LIPEN gene was found to be in a 2.2 kb DNA fragment. The nucleotide sequence of a 1.9 kb segment encoding the LIPEN was determined. This segment showed an open reading frame which would encode a polypeptide of 310 amino acids. The amino acid sequence of this LIPEN gene product has strong homology with those of the Bacillus cereus beta-lactamase III and Bacillus licheniformis penicillinase.

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References

    1. Nucleic Acids Res. 1981 Jan 24;9(2):309-21 - PubMed
    1. J Bacteriol. 1987 Feb;169(2):579-86 - PubMed
    1. Nucleic Acids Res. 1981 Jun 11;9(11):2577-88 - PubMed
    1. J Bacteriol. 1986 Jun;166(3):728-32 - PubMed
    1. Biochemistry. 1983 Sep 27;22(20):4652-6 - PubMed

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