Notch signaling controls chondrocyte hypertrophy via indirect regulation of Sox9

Anat Kohn¹, Timothy P Rutkowski¹, Zhaoyang Liu², Anthony J Mirando³, Michael J Zuscik⁴, Regis J O'Keefe⁵, Matthew J Hilton⁶

Affiliations

¹ Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA ; Department of Biomedical Genetics, University of Rochester Medical Center , Rochester, NY 14642, USA.
² Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA ; Department of Biology, University of Rochester , Rochester, NY 14642, USA.
³ Department of Orthopaedic Surgery, Duke Orthopaedic Cellular, Developmental, and Genome Laboratories, Duke University School of Medicine , Durham, NC 27710, USA.
⁴ Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA.
⁵ Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA ; Department of Orthopaedic Surgery, Washington University School of Medicine , St. Louis, MO 63110, USA.
⁶ Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA ; Department of Orthopaedic Surgery, Duke Orthopaedic Cellular, Developmental, and Genome Laboratories, Duke University School of Medicine , Durham, NC 27710, USA.

PMID: 26558140
PMCID: PMC4640428
DOI: 10.1038/boneres.2015.21

Notch signaling controls chondrocyte hypertrophy via indirect regulation of Sox9

Anat Kohn et al. Bone Res. 2015.

. 2015 Aug 11:3:15021.

doi: 10.1038/boneres.2015.21. eCollection 2015.

Authors

Anat Kohn¹, Timothy P Rutkowski¹, Zhaoyang Liu², Anthony J Mirando³, Michael J Zuscik⁴, Regis J O'Keefe⁵, Matthew J Hilton⁶

Affiliations

¹ Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA ; Department of Biomedical Genetics, University of Rochester Medical Center , Rochester, NY 14642, USA.
² Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA ; Department of Biology, University of Rochester , Rochester, NY 14642, USA.
³ Department of Orthopaedic Surgery, Duke Orthopaedic Cellular, Developmental, and Genome Laboratories, Duke University School of Medicine , Durham, NC 27710, USA.
⁴ Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA.
⁵ Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA ; Department of Orthopaedic Surgery, Washington University School of Medicine , St. Louis, MO 63110, USA.
⁶ Department of Orthopaedics and Rehabilitation, The Center for Musculoskeletal Research, University of Rochester Medical Center , Rochester, NY 14642, USA ; Department of Orthopaedic Surgery, Duke Orthopaedic Cellular, Developmental, and Genome Laboratories, Duke University School of Medicine , Durham, NC 27710, USA.

PMID: 26558140
PMCID: PMC4640428
DOI: 10.1038/boneres.2015.21

Abstract

RBPjk-dependent Notch signaling regulates both the onset of chondrocyte hypertrophy and the progression to terminal chondrocyte maturation during endochondral ossification. It has been suggested that Notch signaling can regulate Sox9 transcription, although how this occurs at the molecular level in chondrocytes and whether this transcriptional regulation mediates Notch control of chondrocyte hypertrophy and cartilage development is unknown or controversial. Here we have provided conclusive genetic evidence linking RBPjk-dependent Notch signaling to the regulation of Sox9 expression and chondrocyte hypertrophy by examining tissue-specific Rbpjk mutant (Prx1Cre;Rbpjk(f/f) ), Rbpjk mutant/Sox9 haploinsufficient (Prx1Cre;Rbpjk(f/f);Sox9(f/+) ), and control embryos for alterations in SOX9 expression and chondrocyte hypertrophy during cartilage development. These studies demonstrate that Notch signaling regulates the onset of chondrocyte maturation in a SOX9-dependent manner, while Notch-mediated regulation of terminal chondrocyte maturation likely functions independently of SOX9. Furthermore, our in vitro molecular analyses of the Sox9 promoter and Notch-mediated regulation of Sox9 gene expression in chondrogenic cells identified the ability of Notch to induce Sox9 expression directly in the acute setting, but suppresses Sox9 transcription with prolonged Notch signaling that requires protein synthesis of secondary effectors.

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Figures

**Figure 1**
Loss of RBPjk leads to inappropriate expression of SOX9 in hypertrophic chondrocytes. Immunohistochemistry for SOX9 in control (a) and *Prx1Cre;Rbpjk^f/f*(b) E14.5 humerus sections.

**Figure 2**
Notch regulates the onset of chondrocyte hypertrophy via *Sox9*. (a) Histological analyses of control (a1), *Prx1Cre;Rbpjk^f/f* (a2), *Prx1Cre;Rbpjk^f/+;Sox9^f/+* (a3), *Prx1Cre;Rbpjk^f/f;Sox9^f/+* (a4), as well as, control (a5) and *Prx1Cre;Sox9^f/+* embryonic tibia sections at E14.5. (b) Quantification of the lengths of the hypertrophic zone, expressed as a percentage of the total length of the element. (b1) WT – wild-type, *Rbpjk* (*Prx1Cre;Rbpjk^f/f*), Dbl Het (*Prx1Cre;Rbpjk^f/+;Sox9^f/+*), RBPJk; Sox9 (*Prx1Cre;Rbpjk^f/f;Sox9^f/+*). (b2) WT – wild-type, Sox9 Het (*Prx1Cre;Sox9^f/+*).

**Figure 3**
Notch regulates the onset of chondrocyte hypertrophy via *Sox9*. Histological and molecular analysis of control, *Prx1Cre;Rbpjk^f/f* and *Prx1Cre;Rbpjk^f/f;Sox9^f/+* embryonic tibia sections at E14.5. ABH/OG staining (**a, b, c**). *In situ* hybridization for markers of chondrocyte maturation – *Indian Hedgehog* (*Ihh*) (**d, e, f**), *Collagen 10a1* (*Col10a1*) (**g, h, i**) and *Matrix Metaloproteinase 13* (*Mmp13*) (**j, k, l**). Yellow arrowheads indicate primary *Ihh* expressing domains. Yellow double-headed arrows indicate *Col10a1* expression domain. Orange circle highlights *Mmp13* expression.

**Figure 4**
Cartilage element length reduction due to *Sox9* haploinsufficiency is rescued by loss of RBPjk-dependent Notch signaling. (a) ABH/OG staining of control (a1), *Prx1Cre;Rbpjk^f/f* (a2), *Prx1Cre;Rbpjk^f/+*;*Sox9^f/+* (a3) and *Prx1Cre;Rbpjk^f/f*;*Sox9^f/+* (a4) E18.5 full length tibia sections. (b) ABH/OG staining of control (b1) and *Prx1Cre*;*Sox9^f/+* (b2) E18.5 tibia sections. (c) Quantification of the size difference of E18.5 tibia sections.

**Figure 5**
Notch regulation of terminal chondrocyte maturation is not likely to be mediated via *Sox9*. (a) Histological and molecular analysis of control, *Prx1Cre;RBPjk^f/f*, *Prx1Cre;RBPjk^f/f*;*Sox9^f/+* embryonic tibia sections at E18.5. ABH/OG staining (a1–a3). Markers of chondrocyte maturation – *Indian Hedgehog* (*Ihh*) (a4–a6), *Collagen 10a1* (*Col10a1*) (a7–a9) and *Matrix Metaloproteinase 13* (*Mmp13*) (**a10**–**a12**) were analyzed by *in situ* hybridization. (b) Immunohistochemistry for SOX9 in control (b1), *Prx1Cre;RBPjk^f/f* (b2), and *Prx1Cre;RBPjk^f/f*;*Sox9^f/+* (b3) embryonic tibia sections at E18.5. Yellow double-headed arrows indicate the length of the SOX9 expressing hypertrophic chondrocyte domain. Red double-headed arrows indicate the total length of the hypertrophic zone.

**Figure 6**
Notch signaling inhibits *Sox9* gene expression via secondary effectors. (a) Diagram for the localization of a RBPjk consensus binding site (yellow box is wild-type sequence and blue box is mutant sequence) and N-box consensus binding site (red box) in the *Sox9* promoter and luciferase constructs. Core consensus sequences are listed in diagram. (b) ATDC5 cells were co-transfected with Flag or NICD1 over-expression vectors and either a wild-type 6.8 kb *Sox9*-Luciferase complex (−6.8 kb WT) or 6.8 kb construct with a mutated RBPjk binding site (−6.8 kb MT). Luciferase levels were measured 24 hours after transfection. (c) ATDC5 cells were co-transfected with Flag or NICD1 over-expression vectors and either a 1 kb, 0.5 kb, or 0.32 kb *Sox9*-Luciferase deletion constructs. Luciferase levels were measured 24 hours after transfection. (d) Quantitative RT-PCR assessing *Hes1* (**d1, d2**), *Hey1* (**d3, d4**), and *Sox9* (**d5, d6**) gene expression in ATDC5 cells at 0-, 2-, 4-, and 8-hour post-culture on IgG versus JAG1 coated plates in the absence (**d1, d3, d5**) or presence (**d2, d4, d6**) of cycloheximide.

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Notch signaling controls chondrocyte hypertrophy via indirect regulation of Sox9

Affiliations

Notch signaling controls chondrocyte hypertrophy via indirect regulation of Sox9

Authors

Affiliations

Abstract

Figures

References

Grants and funding

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Full Text Sources

Other Literature Sources

Research Materials