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. 2015:2015:795656.
doi: 10.1155/2015/795656. Epub 2015 Oct 19.

Analysis of the Oxidative Stress Status in Nonspecific Vaginitis and Its Role in Vaginal Epithelial Cells Apoptosis

Zhaojie Chen  1 , Zhen Zhang  1 , Haiyan Zhang  1 , Beibei Xie  1
Affiliations

Analysis of the Oxidative Stress Status in Nonspecific Vaginitis and Its Role in Vaginal Epithelial Cells Apoptosis

Zhaojie Chen et al. Biomed Res Int. 2015.

Abstract

Nonspecific vaginitis (NSV), also named bacterial vaginosis, is one of the most common genital system diseases in women during their reproductive years. The specific pathogenic mechanism of NSV is not clear yet. Upon the balance alteration, large amount of reactive oxidant species (ROS) is generated and accumulated in the genital tract, and thus resulting in oxidative stress, which has been reported to be an important trigger of mitochondrial pathway cell apoptosis. In this study, the antioxidant secretion level and antioxidant enzyme activity in the vaginal discharge were evaluated to analyze the oxidative status in the vaginal tract of NSV patients. The effect of oxidative stress on the vaginal mucosa epithelial cell apoptosis was then studied. The role of oxidative stress on NSV development was uncovered; thus open new direction for the prevention and treatment of NSV by providing antiradical agents was revealed.

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Figures

Figure 1
Figure 1
LDH activity of mucosa epithelial cells collected from the vagina of healthy women and patients with NSV before and after treatment with metronidazole vaginal effervescent tablets and Vitamin C vaginal tablets. Data was presented as folds change relative control.  ∗Significantly different from control, P < 0.05.
Figure 2
Figure 2
DNA ladder results of mucosa epithelial cells collected from the vagina of healthy women and patients with NSV before and after treatment with metronidazole vaginal effervescent tablets and Vitamin C vaginal tablets. (1) Control, (2) NSV (before), (3) NSV (after), and (4) marker.
Figure 3
Figure 3
Western blot analysis of active caspase 3 in mucosa epithelial cells collected from the vagina of healthy women and patients with NSV before and after treatment with metronidazole vaginal effervescent tablets and Vitamin C vaginal tablets followed by statistical analysis of the optical band density relative to β-actin (relative units).  ∗Significantly different from control, P < 0.05.
Figure 4
Figure 4
Western blot analysis of apoptosis related proteins Bcl-2 and Bax in mucosa epithelial cells collected from the vagina of healthy women and patients with NSV before and after treatment with metronidazole vaginal effervescent tablets and Vitamin C vaginal tablets followed by statistical analysis of the optical band density relative to β-actin (relative units).  ∗Significantly different from control, P < 0.05.
Figure 5
Figure 5
Western blot analysis of cytochrome C in mucosa epithelial cells collected from the vagina of healthy women and patients with NSV before and after treatment with metronidazole vaginal effervescent tablets and Vitamin C vaginal tablets followed by statistical analysis of the optical band density relative to β-actin (relative units).  ∗Significantly different from control, P < 0.05.
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