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Review
. 2016;13(1):83-98.
doi: 10.1586/14789450.2016.1111141. Epub 2015 Nov 11.

Immunocapture strategies in translational proteomics

Claudia Fredolini  1 , Sanna Byström  1 , Elisa Pin  1 , Fredrik Edfors  1 , Davide Tamburro  2 , Maria Jesus Iglesias  1 , Anna Häggmark  1 , Mun-Gwan Hong  1 , Mathias Uhlen  1 , Peter Nilsson  1 , Jochen M Schwenk  1
Affiliations
Review

Immunocapture strategies in translational proteomics

Claudia Fredolini et al. Expert Rev Proteomics. 2016.

Abstract

Aiming at clinical studies of human diseases, antibody-assisted assays have been applied to biomarker discovery and toward a streamlined translation from patient profiling to assays supporting personalized treatments. In recent years, integrated strategies to couple and combine antibodies with mass spectrometry-based proteomic efforts have emerged, allowing for novel possibilities in basic and clinical research. Described in this review are some of the field's current and emerging immunocapture approaches from an affinity proteomics perspective. Discussed are some of their advantages, pitfalls and opportunities for the next phase in clinical and translational proteomics.

Keywords: Antibodies; SRM; immunocapture; mass spectrometry; protein enrichment; sandwich assays.

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Figures

Figure 1.
Figure 1.
Schematic representation of immunocapture strategies discussed in this review. AP-MS: Affinity purification mass spectrometry for interaction studies; FPPA: Forward phase protein array; IC-MS: Immunocapture coupled to mass spectrometry (DDA or DIA); IC-SRM: Immunocapture coupled to SRM; IHC: Immunohistochemistry; RPPA: Reverse phase protein array; SBA: Suspension bead arrays; SIA: Sandwich immunoassay.
See this image and copyright information in PMC

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