doi: 10.1073/pnas.74.3.966.
Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor
- PMID: 265588
- PMCID: PMC430549
- DOI: 10.1073/pnas.74.3.966
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Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor
Proc Natl Acad Sci U S A.
1977 Mar.
Abstract
A number of specific duplex DNA sequences which correspond to varying lengths of the lactose operator region have been synthesized by a combination of chemical and enzymatic methods. Repressor binding studies on these synthetic duplex operator molecules show that all the nucleotides essential for full lactose operator-repressor interactions are included in a 17-nucleotide-long duplex DNA that constitutes the minimal recognition sequence for this DNA-protein interaction.
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