doi: 10.1073/pnas.74.3.975.
Translation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide
- PMID: 265590
- PMCID: PMC430552
- DOI: 10.1073/pnas.74.3.975
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Translation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide
Proc Natl Acad Sci U S A.
1977 Mar.
Abstract
Melittin messenger RNA from queen bee venom glands has been translated in a cell-free system from wheat germ. A product larger than promelittin is formed which has the carboxy-terminal sequence-Gln-Gln-GlyCOOH. Melittin and promelittin from venom glands terminate in -Gln-GlnCONH2. The possible role of the extra glycine residue in the formation of a COOH-terminal amide via a transamidase-like reaction is discussed.
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