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. 2016 Jan;106(1):41-8.
doi: 10.5935/abc.20150137. Epub 2015 Nov 10.

The Effect of Sleep Deprivation on Cardiac Function and Tolerance to Ischemia-Reperfusion Injury in Male Rats

[Article in English, Portuguese]
Sajad Jeddi  1 , Amir Nezami Asl  2 , Alireza Asgari  2 , Asghar Ghasemi  1
Affiliations

The Effect of Sleep Deprivation on Cardiac Function and Tolerance to Ischemia-Reperfusion Injury in Male Rats

[Article in English, Portuguese]
Sajad Jeddi et al. Arq Bras Cardiol. 2016 Jan.

Abstract

Background: Sleep deprivation (SD) is strongly associated with elevated risk for cardiovascular disease.

Objective: To determine the effect of SD on basal hemodynamic functions and tolerance to myocardial ischemia-reperfusion (IR) injury in male rats.

Method: SD was induced by using the flowerpot method for 4 days. Isolated hearts were perfused with Langendorff setup, and the following parameters were measured at baseline and after IR: left ventricular developed pressure (LVDP); heart rate (HR); and the maximum rate of increase and decrease of left ventricular pressure (± dp/dt). Heart NOx level, infarct size and coronary flow CK-MB and LDH were measured after IR. Systolic blood pressure (SBP) was measured at start and end of study.

Results: In the SD group, the baseline levels of LVDP (19%), +dp/dt (18%), and -dp/dt (21%) were significantly (p < 0.05) lower, and HR (32%) was significantly higher compared to the controls. After ischemia, hearts from SD group displayed a significant increase in HR together with a low hemodynamic function recovery compared to the controls. In the SD group, NOx level in heart, coronary flow CK-MB and LDH and infarct size significantly increased after IR; also SD rats had higher SBP after 4 days.

Conclusion: Hearts from SD rats had lower basal cardiac function and less tolerance to IR injury, which may be linked to an increase in NO production following IR.

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Conflict of interest statement

Potential Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Figures

Figure 1
Figure 1
Comparison of changes in systolic blood pressure in control and sleep deprivation groups. Values shown as mean ± SEM.
Figure 2
Figure 2
Change in left ventricular end-diastolic pressure (LVEDP) (ischemic contracture) during experiment. Values shown as mean ± SEM (n = 8 rats)
Figure 3
Figure 3
Recovery of cardiac function after ischemia-reperfusion injury. A. Left ventricular developed pressure (LVDP); B. Heart rate; C. Peak rates of positive changes in left ventricular pressure (+dp/dt); D. Peak rates of negative changes in left ventricular pressure (- dp/dt). Values shown as mean ± SEM (n = 8 rats)
Figure 4
Figure 4
Change in NOx in control and sleep deprivation groups in heart after ischemia. Values shown as mean ± SEM (n = 8 rats)
Figure 5
Figure 5
Change in coronary flow CK-MB (A) and LDH (B) in control and sleep deprivation groups in start of reperfusion. Values shown as mean ± SEM (n = 8 rats)
Figure 6
Figure 6
The alterations of infarct size in heart of control and sleep deprivation rats. Values shown as mean ± SEM (n = 8 rats)
See this image and copyright information in PMC

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