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. 2015 Nov 12;10(11):e0142569.
doi: 10.1371/journal.pone.0142569. eCollection 2015.

Effective Suppression of Methane Emission by 2-Bromoethanesulfonate during Rice Cultivation

Tatoba R Waghmode  1 , Md Mozammel Haque  1 , Sang Yoon Kim  2 , Pil Joo Kim  1   3
Affiliations

Effective Suppression of Methane Emission by 2-Bromoethanesulfonate during Rice Cultivation

Tatoba R Waghmode et al. PLoS One. .

Abstract

2-bromoethanesulfonate (BES) is a structural analogue of coenzyme M (Co-M) and potent inhibitor of methanogenesis. Several studies confirmed, BES can inhibit CH4 prodcution in rice soil, but the suppressing effectiveness of BES application on CH4 emission under rice cultivation has not been studied. In this pot experiment, different levels of BES (0, 20, 40 and 80 mg kg-1) were applied to study its effect on CH4 emission and plant growth during rice cultivation. Application of BES effectively suppressed CH4 emission when compared with control soil during rice cultivation. The CH4 emission rates were significantly (P<0.001) decreased by BES application possibly due to significant (P<0.001) reduction of methnaogenic biomarkers like Co-M concentration and mcrA gene copy number (i.e. methanogenic abunadance). BES significantly (P<0.001) reduced methanogen activity, while it did not affect soil dehydrogenase activity during rice cultivation. A rice plant growth and yield parameters were not affected by BES application. The maximum CH4 reduction (49% reduction over control) was found at 80 mg kg-1 BES application during rice cultivation. It is, therefore, concluded that BES could be a suitable soil amendment for reducing CH4 emission without affecting rice plant growth and productivity during rice cultivation.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Changes in CH4 emission rates with time (A) and total CH4 fluxes (B) under different levels of BES application during rice cultivation.
Error bar indicates standard deviation (n = 3; mean ± SD). Different letters indicate significant difference according to Tukey’s post-hoc test (P<0.05).
Fig 2
Fig 2. Changes of coenzyme M concentrations (A) and mcrA gene copy number (B) in rice paddy soils under different levels of BES application during rice cultivation.
Error bar indicates standard deviation (n = 3; mean ± SD). Different letters indicate significant difference according to the Tukey’s post-hoc test (p<0.05).
Fig 3
Fig 3. Relationships between CH4 emission rates, coenzyme M concentration (A) and mcrA gene copy number (B) during rice cultivation.
See this image and copyright information in PMC

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