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Comment
. 2015 Nov 24;112(47):14404-5.
doi: 10.1073/pnas.1519849112. Epub 2015 Nov 13.

Predictive model of 3D domain formation via CTCF-mediated extrusion

Galip Gürkan Yardımcı  1 , William Stafford Noble  2
Affiliations
Comment

Predictive model of 3D domain formation via CTCF-mediated extrusion

Galip Gürkan Yardımcı et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
(A) Hi-C protocol: A representative DNA loop with six restriction sites is shown as dashed lines, and a cross-linking event is shown with a blue cross. In a typical Hi-C experiment, two proximal regions of DNA are cross-linked, proximal DNA is cleaved, and ends are ligated to each other. The resulting circular fragment is sheared, sequenced from both ends, and mapped back to the genome. Sometimes, a single DNA fragment can fold back and ligate itself, resulting in cyclical Hi-C fragments. (B) Extrusion model: DNA loops are formed by an extrusion complex (blue circle) binding to a locus and beginning to extrude DNA. The complex moves in both directions, extruding DNA until it encounters two CTCF motifs (red). The extruded DNA forms a loop enriched for contacts.
See this image and copyright information in PMC

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