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. 1989 Jul;63(7):3176-9.
doi: 10.1128/JVI.63.7.3176-3179.1989.

N myristylation of the human immunodeficiency virus type 1 gag polyprotein precursor in Saccharomyces cerevisiae

I C Bathurst  1 , N Chester, H L Gibson, A F Dennis, K S Steimer, P J Barr
Affiliations

N myristylation of the human immunodeficiency virus type 1 gag polyprotein precursor in Saccharomyces cerevisiae

I C Bathurst et al. J Virol. 1989 Jul.

Abstract

A semisynthetic gene precisely encoding the 502 amino acids of the human immunodeficiency virus type 1 gag precursor (Pr53gag) was expressed in the yeast Saccharomyces cerevisiae. Amino acid sequence analysis of the recombinant Pr53gag showed that the amino terminus was fully blocked. Labeling of Pr53gag with [3H]myristic acid demonstrated that, as with Pr53gag isolated from virus-infected cells, the yeast-derived protein was demethionylated and N myristylated on glycine, the second amino acid residue.

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