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. 2016 Feb 11;52(12):2477-80.
doi: 10.1039/c5cc08592f. Epub 2015 Nov 17.

Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

Heeseon An  1 , Alexander V Statsyuk
Affiliations

Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

Heeseon An et al. Chem Commun (Camb). .

Abstract

We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.

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