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. 2015 Dec;29(6):500-502.
doi: 10.1016/j.mcp.2015.11.002. Epub 2015 Nov 11.

Thioflavin T as a fluorescence probe for label-free detection of T4 polynucleotide kinase/phosphatase and its inhibitors

Changbei Ma  1 , Shunxin Jin  2 , Haisheng Liu  2 , Kun Xia  2 , Jianhua Tang  2 , Kemin Wang  3 , Jun Wang  4
Affiliations

Thioflavin T as a fluorescence probe for label-free detection of T4 polynucleotide kinase/phosphatase and its inhibitors

Changbei Ma et al. Mol Cell Probes. 2015 Dec.

Abstract

We have developed a new methodology for label-free fluorescence turn-on detection of T4 polynucleotide kinase/phosphatase activity (T4 PNKP) using a Thioflavin T probe. This method is very sensitive with a 0.01 unit/mL limit of detection, which is better than those with labeled fluorophores. Furthermore, T4 PNKP inhibition by the inhibitor heparin is shown, demonstrating the potential to screen suitable inhibitor drugs for T4 PNKP.

Keywords: Label-free; Polynucleotide kinase/phosphatase; Thioflavin T.

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