Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2015 Oct 27:6:166.
doi: 10.3389/fendo.2015.00166. eCollection 2015.

Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies

Yoko Fujita-Yamaguchi  1
Affiliations
Review

Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies

Yoko Fujita-Yamaguchi. Front Endocrinol (Lausanne). .

Abstract

Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules, including subunits. Nowadays, we take the effectiveness and excellence of this technology for granted. This essay will mainly cover the use of affinity chromatography based on my experience.

Keywords: IGF-I; affinity chromatography; insulin; receptors; single-chain antibodies.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Fujita Y, Oishi K, Suzuki K, Imahori K. Purification and properties of an anti-B hemagglutinin produced by Streptomyces sp. Biochemistry (1975) 14:4465–70.10.1021/bi00691a019 - DOI - PubMed
    1. Fujita-Yamaguchi Y, Yoshida A. Purification and characterization of human serum galactosyltransferase (lactose synthetase A protein). J Biol Chem (1981) 256:2701–6. - PubMed
    1. Fujita-Yamaguchi Y, Choi S, Sakamoto Y, Itakura K. Purification of insulin receptor with full binding activity. J Biol Chem (1983) 258:5045–9. - PubMed
    1. LeBon TR, Jacobs S, Cuatrecasas P, Kathuria S, Fujita-Yamaguchi Y. Purification of insulin-like growth factor I receptor from human placental membranes. J Biol Chem (1986) 261:7685–9. - PubMed
    1. LeBon TR, Kasuya J, Paxton RJ, Belfrage P, Hockman S, Manganiello VC, et al. Purification and characterization of guanosine 3’,5’-monophosphate-inhibited low K(m) adenosine 3’,5’-monophosphate phosphodiesterase from human placental cytosolic fractions. Endocrinology (1992) 130:3265–74.10.1210/endo.130.6.1317779 - DOI - PubMed

LinkOut - more resources