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. 2015 Nov;22(6):685-91.
doi: 10.1016/j.sjbs.2015.03.010. Epub 2015 Mar 27.

Effect of acetone extract from stem bark of Acacia species (A. dealbata, A. ferruginea and A. leucophloea) on antioxidant enzymes status in hydrogen peroxide-induced HepG2 cells

Kandhasamy Sowndhararajan  1 , Sunghyun Hong  2 , Jin-Woo Jhoo  2 , Songmun Kim  3 , Nyuk Ling Chin  1
Affiliations

Effect of acetone extract from stem bark of Acacia species (A. dealbata, A. ferruginea and A. leucophloea) on antioxidant enzymes status in hydrogen peroxide-induced HepG2 cells

Kandhasamy Sowndhararajan et al. Saudi J Biol Sci. 2015 Nov.

Abstract

Acacia species are multipurpose trees, widely used in the traditional systems of medicine to treat various ailments. The major objective of the present study was to determine the gene expression of enzymatic antioxidants by acetone extract from the stem bark of three Acacia species (Acacia dealbata, Acacia ferruginea and Acacia leucophloea) in hydrogen peroxide (H2O2)-induced human hepatoma (HepG2) cells. The expression of antioxidant enzymes such as superoxide dismutase containing copper-zinc (CuZnSOD)/manganese (MnSOD), catalase (CAT) and glutathione peroxidase (GPx) in HepG2 cells was evaluated by real-time PCR. The results of antioxidant enzyme expression in real-time PCR study revealed that the H2O2 (200 μM) challenged HepG2 cells reduced the expression of enzymes such as SOD, GPx and CAT. However, the cells pre-treated with acetone extracts of all the three Acacia species significantly (P > 0.05) up-regulated the expression of antioxidant enzymes in a concentration dependent manner (25, 50 and 75 μg/mL). In conclusion, the findings of our study demonstrated that the acetone extract of Acacia species effectively inhibited H2O2 mediated oxidative stress and may be useful as a therapeutic agent in preventing oxidative stress mediated diseases.

Keywords: Acacia; Antioxidant enzyme; Catalase; HepG2 cells; Superoxide dismutase.

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Figures

Figure 1
Figure 1
Effect of different concentrations (25, 50 and 75 μg/mL) of acetone extracts of Acacia barks on CuZnSOD activity in H2O2-stimulated HepG2 cells. ADA, A. dealbata; AFA, A. ferruginea; ALA, A. leucophloea. Values are mean of three replicate determinations (n = 3) ± standard deviation. Bars having different letters are significantly different (P < 0.05).
Figure 2
Figure 2
Effect of different concentrations (25, 50 and 75 μg/mL) of acetone extracts of Acacia barks on MnSOD activity in H2O2-stimulated HepG2 cells. ADA, A. dealbata; AFA, A. ferruginea; ALA, A. leucophloea. Values are mean of three replicate determinations (n = 3) ± standard deviation. Bars having different letters are significantly different (P < 0.05).
Figure 3
Figure 3
Effect of different concentrations (25, 50 and 75 μg/mL) of acetone extracts of Acacia barks on CAT activity in H2O2-stimulated HepG2 cells. ADA, A. dealbata; AFA, A. ferruginea; ALA, A. leucophloea. Values are mean of three replicate determinations (n = 3) ± standard deviation. Bars having different letters are significantly different (P < 0.05).
Figure 4
Figure 4
Effect of different concentrations (25, 50 and 75 μg/mL) of acetone extracts of Acacia barks on GPx activity in H2O2-stimulated HepG2 cells. ADA, A. dealbata; AFA, A. ferruginea; ALA, A. leucophloea. Values are mean of three replicate determinations (n = 3) ± standard deviation. Bars having different letters are significantly different (P < 0.05).
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References

    1. Bak M.J., Jun M., Jeong W.S. Antioxidant and hepatoprotective effects of the red ginseng essential oil in H2O2-treated HepG2 cells and CCl4-treated mice. Int. J. Mol. Sci. 2012;13:2314–2330. - PMC - PubMed
    1. Bak M.J., Jeong J.H., Kang H.S., Jin K.S., Jun M., Jeong W.S. Stimulation of activity and expression of antioxidant enzymes by solvent fractions and isolated compound from Cedrela sinensis leaves in HepG2 cells. J. Med. Food. 2011;14:405–412. - PubMed
    1. Borneo R., Leon A., Aguirre A., Ribotta P., Cantero J. Antioxidant capacity of medicinal plants from the province of Cordoba (Argentina) and their in vitro testing in a model food system. Food Chem. 2009;112:664–670.
    1. Carvalho A.C., Franklin G., Dias A.C.P., Lima C.F. Methanolic extract of Hypericum perforatum cells elicited with Agrobacterium tumefaciens provides protection against oxidative stress induced in human HepG2 cells. Ind. Crops Prod. 2014;59:177–183.
    1. Chiu Y.W., Lo H.J., Huang H.Y., Chao P.Y., Hwang J.M., Huang P.Y., Huang S.J., Liu J.Y., Lai T.J. The antioxidant and cytoprotective activity of Ocimum gratissimum extracts against hydrogen peroxide-induced toxicity in human HepG2 cells. J. Food Drug Anal. 2013;21:253–260.

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