Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016;20(2):84-90.
doi: 10.7508/ibj.2016.02.003. Epub 2015 Nov 16.

Identification of Sequence Variation in the Apolipoprotein A2 Gene and Their Relationship with Serum High-Density Lipoprotein Cholesterol Levels

Fatemeh Bandarian  1   2 , Maryam Sadat Daneshpour  3 , Mehdi Hedayati  3 , Mohsen Naseri  4 , Fereidoun Azizi  3
Affiliations

Identification of Sequence Variation in the Apolipoprotein A2 Gene and Their Relationship with Serum High-Density Lipoprotein Cholesterol Levels

Fatemeh Bandarian et al. Iran Biomed J. 2016.

Abstract

Background: Apolipoprotein A2 (APOA2) is the second major apolipoprotein of the high-density lipoprotein cholesterol (HDL-C). The study aim was to identify APOA2 gene variation in individuals within two extreme tails of HDL-C levels and its relationship with HDL-C level.

Methods: This cross-sectional survey was conducted on participants from Tehran Glucose and Lipid Study (TLGS) at Research Institute for Endocrine Sciences, Tehran, Iran from April 2012 to February 2013. In total, 79 individuals with extreme low HDL-C levels (≤5th percentile for age and gender) and 63 individuals with extreme high HDL-C levels (≥95th percentile for age and gender) were selected. Variants were identified using DNA amplification and direct sequencing.

Results: Screen of all exons and the core promoter region of APOA2 gene identified nine single nucleotide substitutions and one microsatellite; five of which were known and four were new variants. Of these nine variants, two were common tag single nucleotide polymorphisms (SNPs) and seven were rare SNPs. Both exonic substitutions were missense mutations and caused an amino acid change. There was a significant association between the new missense mutation (variant Chr.1:16119226, Ala98Pro) and HDL-C level.

Conclusion: None of two common tag SNPs of rs6413453 and rs5082 contributes to the HDL-C trait in Iranian population, but a new missense mutation in APOA2 in our population has a significant association with HDL-C.

Keywords: Apolipoprotein A-II; Genes; Hyperlipidemia; Mutation; Polymorphism.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
The sequence of a known (A) and new (B) variant in APOA2 sequence. Arrows show the location of variant
See this image and copyright information in PMC

References

    1. Kareinen A, Viitanen L, Halonen P, Lehto S, Laakso M. Cardiovascular risk factors associated with insulin resistance cluster in families with early-onset coronary heart disease. Arteriosclerosis, thrombosis, and vascular biology. 2001;21(8):1346–1352. - PubMed
    1. Tabatabaei-Malazy O, Qorbani M, Samavat T, Sharifi F, Larijani B, Fakhrzadeh H. Prevalence of dyslipidemia in iran: A systematic review and meta-analysis study. International journal of preventive medicine. 2014;5(4):373–393. - PMC - PubMed
    1. Weisgraber KH, Mahley RW. Apoprotein (E--A-II) complex of human plasma lipoproteins. I. Characterization of this mixed disulfide and its identification in a high density lipoprotein subfraction. The journal of biological chemistry. 1978;253(17):6281–6288. - PubMed
    1. Borghini I, James RW, Blatter MC, Pometta D. Distribution of apolipoprotein e between free and a-ii complexed forms in very-low- and high-density lipoproteins: Functional implications. Biochimica et biophysica acta. 1991;1083(2):139–146. - PubMed
    1. Tailleux A, Duriez P, Fruchart JC, Clavey V. Apolipoprotein A-II, HDL metabolism and atherosclerosis. Atherosclerosis. 2002;164(1):1–13. - PubMed

Publication types