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Comparative Study
. 2015 Nov;160(1):123-8.
doi: 10.1007/s10517-015-3112-5. Epub 2015 Nov 22.

Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming

A S Vdovin  1 , A Yu Lupatov  1 , I V Kholodenko  2 , K N Yarygin  1
Affiliations
Comparative Study

Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming

A S Vdovin et al. Bull Exp Biol Med. 2015 Nov.

Abstract

Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 10(5) cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60.

Keywords: differentiation; induced pluripotent stem cells; reprogramming; transduction; transfection.

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